Re-evaluation of the function of the male specific lethal complex in Drosophila
Re-evaluation of the function of the male specific lethal complex in Drosophila
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DOI：
10.1016/j.jgg.2011.07.001
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发表时间：
2011-08-01
影响因子：
5.9
通讯作者：
Sanyal, Abhijit
中科院分区：
文献类型：
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作者：
Birchler, James;Sun, Lin;Sanyal, Abhijit
一组蛋白质和非编码 RNA，称为雄性特异性致死 (MSL) 复合物，存在于果蝇的雄性 X 染色体上，并被认为负责该染色体的剂量补偿，即其表达上调，使其等于雌性两条 X 染色体的表达。这一假设是根据剂量补偿鲜为人知的方面进行评估的，例如具有三个 X 染色体的元女性也具有与正常女性相同的表达，这需要每个基因拷贝的下调。此外，当该复合物在女性中异位表达或专门针对男性中的报告基因时，与其相关的基因或靶标的表达不会增加。这些观察结果与 MSL 复合物条件剂量补偿的假设不一致。描述了可以解释这些观察结果的综合。
A set of proteins and noncoding RNAs, referred to as the male specific lethal (MSL) complex, is present on the male X chromosome in Drosophila and has been postulated to be responsible for dosage compensation of this chromosome e the up-regulation of its expression to be equal to that of two X chromosomes in females. This hypothesis is evaluated in view of lesser known aspects of dosage compensation such as the fact that metafemales with three X chromosomes also have equal expression to normal females, which would require a down-regulation of each gene copy. Moreover, when this complex is ectopically expressed in females or specifically targeted to a reporter in males, there is no increase in expression of the genes or targets with which it is associated. These observations are not consistent with the hypothesis that the MSL complex conditions dosage compensation. A synthesis is described that can account for these observations.