Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography.
Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography.
复制标题
通过电子显微镜和断层扫描探测细菌分裂体的膜相关细胞骨架寡聚物。
DOI：
10.1007/978-1-0716-3491-2_2
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Margolin,William
中科院分区：
文献类型：
--
作者：
Hu,Bo;Margolin,William
包括大肠杆菌在内的许多细菌的细胞分裂机制或“分裂体”含有微管蛋白（FtsZ）和肌动蛋白（FtsA）的同源物，它们相互作用以促进隔肽聚糖的合成。FtsA寡聚体作为一个轨道的拴系动力学milling FtsZ原丝细胞质膜的重要作用。其他细菌细胞骨架寡聚体如MreB也在膜上组装并沿着移动。这些低聚物在体外膜上的结构可以模拟它们在细胞中的行为。在这里，我们描述了一个协议，可视化FtsA寡聚体结构的膜和它们的相互作用与FtsZ原丝使用负染色透射电子显微镜沿着与断层扫描。
The cell division machinery or “divisome” of many bacteria, includingEscherichia coli, contains homologs of tubulin (FtsZ) and actin (FtsA) that interact with each other to promote the synthesis of septal peptidoglycan. FtsA oligomers have an essential role as a track for tethering dynamically treadmilling FtsZ protofilaments to the cytoplasmic membrane. Other bacterial cytoskeletal oligomers such as MreB also assemble on and move along the membrane. Structures of these oligomers on membranes in vitro may mimic their behavior in the cell. Here, we describe a protocol to visualize FtsA oligomeric structures on membranes and their interactions with FtsZ protofilaments using negative stain transmission electron microscopy along with tomography.