Advantages and limitations of 1-palmitoyl-2-[[2-[4- (6-phenyl-trans-1,3,5-hexatrienyl)phenyl]ethyl]carbonyl]-3- sn-phosphatidylcholine as a fluorescent membrane probe.
Advantages and limitations of 1-palmitoyl-2-[[2-[4- (6-phenyl-trans-1,3,5-hexatrienyl)phenyl]ethyl]carbonyl]-3- sn-phosphatidylcholine as a fluorescent membrane probe.
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1-棕榈酰-2-[[2-[4-(6-苯基-反式-1,3,5-六三烯基)苯基]乙基]羰基]-3-sn-磷脂酰胆碱作为荧光膜探针的优点和局限性。
DOI：
10.1021/bi00343a022
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Lentz,BR
中科院分区：
文献类型：
--
作者：
Parente,RA;Lentz,BR
北卡罗来纳大学生物化学与营养系，教堂山，北卡罗来纳州 27514 收到 1985 年 1 月 3 日 摘要：我们研究了 l-棕榈酰-2-[[2-[4-(6-苯基-tran^-l, 3, 5-六三烯基)苯基]乙基]羰基]-3-5 «-磷脂酰胆碱 (DPHpPC) 在合成多层膜中的行为磷脂酰胆碱囊泡。这种荧光磷脂具有与其母体荧光团二苯基己三烯 (DPH) 相似的光物理特性。 DPHpPC 优先分配到液相脂质中 (K!/s= 3.3)，并且通过荧光各向异性检测到的相变温度低于通过差示扫描量热法检测到的相变温度。对具有不同磷脂与探针比率的样品中双层相变的量热测量表明，膜相变温度（0.1-0.2℃）发生了非常轻微的变化，并且转变宽度没有显示出可测量的变化。尽管如此，探针荧光特性的测量表明，DPHpPC 破坏了膜中的局部环境，甚至可能在磷脂相变下方诱导富含探针的局部区域受到干扰。稳态荧光各向异性、极限各向异性、微分正切和旋转速率的温度曲线与低于主要脂质相变温度的 DPH 的温度曲线相似，但表明高于脂质相变温度时旋转运动受到更多限制。对于 DPH，DPHpPC 的荧光衰减可以通过 DPPC 相变上方和下方的单指数或双指数来描述。选择似乎取决于样品的处理。 DPHpPC 的强度加权平均寿命比 DPH 大约短 1.5 ns。总之，DPHpPC 的测量特性及其类脂结构使其成为膜结构和动力学的有力探针。
Department of Biochemistry and Nutrition, University of North Carolina, Chapel Hill, North Carolina 27514 Received January 3, 1985 abstract: We have investigated the behavior of l-palmitoyl-2-[[2-[4-(6-phenyl-tran^-l, 3, 5-hexatri-enyl) phenyl] ethyl] carbonyl]-3-5 «-phosphatidylcholine (DPHpPC) in synthetic, multilamellar phosphatidylcholine vesicles. This fluorescent phospholipid has photophysical properties similar to its parent fluorophore, diphenylhexatriene (DPH). DPHpPC preferentially partitioned into fluid phase lipid (K!/s= 3.3) and reported a lower phase transition temperature as detected by fluorescence anisotropy than that ooserved by differential scanning calorimetry. Calorimetric measurements of the bilayer phase transition in samples having different phospholipid to probe ratios demonstrated very slight changes in membrane phase transition temperature (0.1—0.2 C) and showed no measurable change in transition width. Nonetheless, measurements of probe fluorescence properties suggested that DPHpPC disrupts its local environment in the membrane and may even induce perturbed probe-rich local domains below the phospholipid phase transition. Temperature profiles of steady-state fluorescence anisotropy, limiting anisotropy, differential tangent, and rotational rate were similar to those of DPH below the main lipid phase transition but indicated more restricted rotational motion above the lipid phase transition temperature. As for DPH, the fluorescence decay of DPHpPC could be described by either a single or double exponential both above and below the DPPC phase transition. The choice seemed dependent on the treatment of the sample. The intensity-weighted average lifetime of DPHpPC was roughly 1.5 ns shorter than that of DPH. In summary, the measured properties of DPHpPC and its lipid-like structure make it a powerful probe of membranestructure and dynamics.