Salamanders in Regeneration Research
Salamanders in Regeneration Research
复制标题
DOI：
10.1007/978-1-4939-2495-0
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Anoop Kumar;A. Simon
中科院分区：
文献类型：
--
作者：
Anoop Kumar;A. Simon
实验生物学中许多最基本的发现，例如胚胎组织者、神经元特异性、神经引导和 DNA 转录单位，都源自蝾螈研究。蝾螈是唯一能够在成年后反复再生整个肢体的四足动物，而且它们还表现出最广泛的其他复杂组织和器官的再生能力。这些动物构成了了解脊椎动物丢失或受损结构的形态和功能恢复的关键过程的独特模型。本卷重点关注蝾螈生物学的这一特定方面，该领域在过去 10-15 年中获得了新的动力，部分原因是人们对干细胞和再生医学的普遍兴趣。使用“蝾螈”和“再生”这两个词对谷歌学术进行综合搜索显示，每年发表的论文数量稳步增长，2001年至2013年间增长了140%，在此期间发表的文章超过10,000篇。实验室最常研究的蝾螈在一般生理学、生命周期、再生谱以及替代结构形成机制方面存在相当大的差异。本书的第一部分概述了在实验室中维持最常用蝾螈物种的最佳实践和条件。以下两部分的章节分别描述了体内和体外的实验操作。其中包括针对各种结构的方法，从四肢到心脏再到大脑。最后两个部分涉及转基因生物和基因组数据库挖掘工具。这些章节说明了最新技术发展的繁荣，为使用最现代的分子工具了解蝾螈再生提供了新的平台。在本书的方法章节之前，杰里米·布洛克斯 (Jeremy Brockes) 从系统发育和进化的角度发表了一篇关于蝾螈再生的鼓舞人心的文章，他为振兴这一研究领域做出了巨大贡献。最后，我们感谢所有同事花费宝贵的时间和精力，为制作这本全面的方法章节集提供了所有更详细的细节。我们希望这个集合对所有已经致力于蝾螈再生的人以及那些刚刚考虑深入研究这个有趣问题的人有用。
Many of the most fundamental discoveries in experimental biology, such as the embryonic organizers, neuronal specificity, nerve guidance, and units of DNA transcription, originate from salamander research. Salamanders are the only tetrapods capable of repeatedly regenerating entire limbs as adults, and they also display the widest range of regeneration capacities of other complex tissues and organs. These animals constitute unique models for understanding critical processes underlying morphological and functional restoration of lost or damaged structures in vertebrates. The present volume focuses on this particular aspect of salamander biology, which has gained new momentum during the past 10–15 years, partly due to the general interest in stem cells and regenerative medicine. A combined search on Google scholar using the terms “salamander” and “regeneration” shows a steady growth in the number of yearly publications with a 140% increase between 2001 and 2013, resulting in more than 10,000 published articles during this period. There are considerable variations among the most commonly studied salamanders in the laboratory in terms of their general physiology, life cycle, regeneration spectrum, and also mechanisms by which replacement structures form. The first part of the book outlines the best practices and conditions for maintaining the most commonly used salamander species in the laboratory. The chapters of the two following parts describe experimental manipulations in vivo and in vitro, respectively. These include methods targeting a wide variety of structures, ranging from the limb to the heart and to the brain. The two final sections deal with genetically modified organisms and tools for mining in the genomic databases. These chapters illustrate the boom of recent technical developments, which provide new platforms for understanding salamander regeneration using the most modern molecular tools. The methods chapters of this book are preceded by an inspiring essay on salamander regeneration from phylogenetic and evolutionary perspectives by Jeremy Brockes, who has greatly contributed to revitalize this research field. Finally, we thank all the colleagues for their invaluable time and efforts to provide with all the finer details to produce this comprehensive collection of methods chapters. We hope that this collection will be useful to all, who already are devoting our activities to salamander regeneration, as well as for those who are just considering to dwell on to this intriguing problem.