CLONING OF THE HUMAN PARVOVIRUS-B19 GENOME AND STRUCTURAL-ANALYSIS OF ITS PALINDROMIC TERMINI
CLONING OF THE HUMAN PARVOVIRUS-B19 GENOME AND STRUCTURAL-ANALYSIS OF ITS PALINDROMIC TERMINI
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DOI：
10.1016/0042-6822(90)90205-6
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发表时间：
1990-03-01
期刊：
影响因子：
3.7
通讯作者：
SIEGL, G
中科院分区：
文献类型：
--
作者：
DEISS, V;TRATSCHIN, JD;SIEGL, G
我们描述了人细小病毒B19全长5，6kb单链DNA基因组在细菌质粒中的分子克隆。重组质粒DNA在大肠杆菌JC8111中得到稳定扩增，但在HB101细胞中未得到稳定扩增。对克隆DNA的序列分析表明，基因组两端的383个核苷酸是相同的反向重复序列。该重复序列的末端365个核苷酸代表了一个不完美的回文，它可能会折叠形成一个发夹结构。发夹的序列出现在两种不同的配置中，这两种配置是相关的，因为一种是另一种的倒置补语。到目前为止，已经在所有分析的细小病毒中发现了这种末端发夹的替代构型，并被称为翻转和翻转。
We describe the molecule cloning of ther entire 5,6-kb single-stranded DNA genome of the human parvovirus B19 in bacterial plasmids. Stable amplification of the recombinant plasmid DNA was acheived in Escherichia coli JC8111 but not in HB101 cells. Sequence analysis of the cloned DNA shows that the terminal 383 nucleotides at each end of the genome are identical inverted repeats. The distal 365 nucleotides of the repeat represent an imperfect palindrome which presumably folds over to form a hairpin structure. The sequence of the hairpin occurs in two distinct configurations which are related in that one is the inverted complement of the other. Such alternative configurations of the terminal hairpins have been found for all parvoviruses analyzed so far and are referred to as flip and flop.