Effects of vanadium upon polyl:C-induced responses in rat lung and alveolar macrophages.
Effects of vanadium upon polyl:C-induced responses in rat lung and alveolar macrophages.
复制标题
钒对 Polyl:C 诱导的大鼠肺和肺泡巨噬细胞反应的影响。
DOI：
10.1080/00984109708984046
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Zelikoff,JT
中科院分区：
文献类型：
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作者：
Cohen,MD;Becker,S;Devlin,R;Schlesinger,RB;Zelikoff,JT
暴露于钒（V）的微生物随后表现出对感染性微生物的抵抗力下降。我们早期对吸入职业相关水平的V（如偏钒酸铵，NH 4V 03）的大鼠的研究表明，肺巨噬细胞（PAM）的几种新生/诱导功能降低。在本研究中，V暴露大鼠进行了检查，以确定是否也可能发生一些相同的影响在原位。大鼠经鼻暴露于空气或2 mgV/m3（以NH_4VO_3计）中，连续4d，8h/d，24 h后分别经鼻腔滴入多聚肌苷酸、多聚胞苷酸或生理盐水。灌注polyhC后灌洗肺细胞/液体的分析表明，总的可灌洗细胞/中性粒细胞数量和蛋白质水平，而显着升高的两个暴露组（以及在盐水处理的V暴露大鼠），总是在V暴露主机。暴露于V也影响白细胞介素6（IL-6）和干扰素γ（IFNγ）的诱导产生，但显然不影响肿瘤坏死因子-α（TNFα）或IL-1的诱导产生。尽管在两个暴露组中聚乙二醇：C诱导灌洗液IL-6和IFNγ水平显著增加，但V暴露大鼠中的水平更高。如果计算相对于总灌洗蛋白，然而，V暴露大鼠产生的细胞因子显着减少。聚乙二醇：C滴注后，有没有显着的基础或刺激超氧阴离子产生的差异合并灌洗细胞或PAM具体。与V暴露的大鼠，合并的细胞恢复后24小时，盐水滴注显示减少生产（在这两种情况下）相比，空气对照细胞; PAM特异性生产的影响，只有刺激后。在两个暴露组中，聚乙二醇：C引起恢复的细胞中超氧化物的产生减少。虽然不太明显，
Hosts exposed to vanadium (V) display a subsequent decrease in their resistance to infectious microorganisms. Our earlier studies with rats inhaling occupationally relevant levels of V (as ammonium metavanadate, NH4V03) indicated that several nascent/inducible functions of pulmonary macrophages (PAM) were reduced. In the present study, V-exposed rats were examined to determine whether some of the same effects might also occur in situ. Rats were exposed nose-only to air or 2 mg V/m3 (as NH4VO3) for 8 h/d for 4 d, followed, 24 h later, by intratracheal (it) instillation of polyinosinic.polycytidilic acid (polyhC) or saline. Analysis of lavaged lung cells/fluids after polyhC instillation indicated that total lavageable cell/neutrophil numbers and protein levels, while significantly elevated in both exposure groups (as well as in saline-treated V-exposed rats), were always greater in V-exposed hosts. Exposure to V also affected the inducible production of interleukin 6 (IL-6) and interferon γ (IFNγ), but apparently not that of tumor necrosis factor-α(TNFα) or IL-1. Although polyl:C induced significant increases in lavage fluid IL-6 and IFNγ levels in both exposure groups, levels were greater in V-exposed rats. If calculated with respect to total lavaged protein, however, V-exposed rats produced significantly less cytokine. Following polyl:C instillation, there were no marked exposure-related differences in basal or stimulated superoxide anion production by pooled lavaged cells or PAM specifically. With V-exposed rats, pooled cells recovered 24 h after saline instillation displayed reduced production (in both cases) compared to the air control cells; PAM-specific production was affected only after stimulation. In both exposure groups, polyl:C caused decreased superoxide production in recovered cells. Though less apparent with pooled