Counting Bacteria Using Functionalized Gold Nanoparticles as the Light-Scattering Reporter
Counting Bacteria Using Functionalized Gold Nanoparticles as the Light-Scattering Reporter
复制标题
使用功能化金纳米颗粒作为光散射报告基因进行细菌计数
DOI：
10.1021/ac302471c
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发表时间：
2012-11-20
影响因子：
7.4
通讯作者：
Li, Na
中科院分区：
文献类型：
--
作者：
Xu, Xiao;Chen, Yang;Li, Na
建立了一种基于细菌和金纳米粒子（AuNP）报告分子的暗场光散射成像的细菌计数方法。市售DH 5 α E。以大肠杆菌为模型菌，验证了该方法的可行性。使用抗体缀合的AuNPs，简单的样品处理和靶E.大肠杆菌菌株识别可在15-30 min内完成，检测限为2-6 × 10（4）菌落形成单位/毫升（CFU/mL）。通过使用90纳米金纳米粒子作为光散射信号报告分子，可以使用低成本仪器（例如入门级暗场显微镜装置和普通钨灯作为光源）轻松进行细菌识别和计数。还开发了自动图像分析算法，以促进稳健和快速的细菌计数。水、牛奶和果汁样品的初步分析结果表明，这种简单、快速、成本效益高的方法可以很容易地用于常规细菌检测。
A simple and rapid bacterial counting method was developed based on dark-field light-scattering imaging of bacteria and gold nanoparticle (AuNP) reporter simultaneously. Commercially available DH5 alpha E. coli strain was used as the model bacterium to demonstrate the feasibility of the proposed method. With antibody-conjugated AuNPs, the simple sample treatment and target E. coli strain recognition can be finished within 15-30 min, with a detection limit of 2-6 x 10(4) colony forming unit per milliliter (CFU/mL). By using 90 nm AuNPs as the light-scattering signal reporter, the bacterial recognition and counting can be easily performed with low-cost instrumentation such as an entry-level dark-field microscope setup and a common tungsten lamp as the light source. An automatic image analysis algorithm was also developed to facilitate robust and fast bacterial counting. The preliminary results of water, milk, and fruit juice sample analysis showed that this simple, fast, and cost-effective method can be easily adopted for routine bacterial detection.