EVALUATION OF MYCOBACTERIAL ANTIGENS IN AN ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) FOR THE SERODIAGNOSIS OF TUBERCULOSIS
EVALUATION OF MYCOBACTERIAL ANTIGENS IN AN ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) FOR THE SERODIAGNOSIS OF TUBERCULOSIS
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DOI：
10.1099/00222615-18-3-309
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发表时间：
1984-01-01
影响因子：
3
通讯作者：
DANIEL, TM
中科院分区：
文献类型：
--
作者：
BENJAMIN, RG;DEBANNE, SM;DANIEL, TM
本文比较了五种分枝杆菌抗原在结核病血清学诊断中的作用。所研究的抗原是结核分枝杆菌的未加热无菌培养滤液、结核分枝杆菌的结核菌素纯化蛋白衍生物（PPD）。结核分枝杆菌（PPDa）胞浆蛋白抗原5和6。结核分枝杆菌和PPD。kansasii（PPDk）。多因素方差分析显示，结核病患者和对照组中，ELISA中每种抗原获得的几何平均滴度存在显著差异。ELISA结果与5种抗原之间的协变具有高度的相互依赖性。对受试者操作特性的分析表明，用抗原5获得了最准确的试验;结核抗原6和M.结核菌培养滤液的准确性从高到低依次降低。
Five mycobacterial antigens were compared in an ELISA for the serodiagnosis of tuberculosis. The antigens studied were an unheated sterile culture filtrate of Mycobacterium tuberculosis, tuberculin purified protein derivative (PPD) from M. tuberculosis (PPDa), purified cytoplasmic protein antigens 5 and 6 from M. tuberculosis and a PPD prepared from M. kansasii (PPDk). Multivariate analysis of variance showed that geometric mean titers obtained with each of the antigens in ELISA were significantly different in tuberculosis patients and in control groups. The covariation of the ELISA results with the 5 antigens was highly interdependent. Analysis of receiver operating characteristics revealed that the most accurate test was obtained with antigen 5; PPDa, M. tuberculosis antigen 6 and M. tuberculosis culture filtrate were, in descending order, less accurate.