Polydiacetylene Liposome Microarray toward Facile Measurement of Platelet Activation in Whole Blood.
Polydiacetylene Liposome Microarray toward Facile Measurement of Platelet Activation in Whole Blood.
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DOI：
10.1021/acssensors.1c01167
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发表时间：
2021-07
期刊：
影响因子：
8.9
通讯作者：
Deokwon Seo;T. Major;D. Kang;Sungbaek Seo;Kangwon Lee;R. Bartlett;Jinsang Kim
中科院分区：
文献类型：
--
作者：
Deokwon Seo;T. Major;D. Kang;Sungbaek Seo;Kangwon Lee;R. Bartlett;Jinsang Kim
在临床医学中，血小板活化的简单测量的必要性已经增加，以调节用于在急性情况下具有临床结果的患者的抗血小板药物的适当剂量，所述急性情况例如心绞痛、中风或外周血管疾病或涉及血管成形术或冠状动脉血栓溶解的程序。我们开发了一种自信号聚丁二炔（PDA）脂质体微阵列检测活化血小板从全血样品中在一个单一的步骤。选择针对血小板结合的纤维蛋白原的特异性抗体9 F9抗体，并将其缀合至PDA脂质体微阵列以定量纤维蛋白原结合的血小板。当引入全血中的活化血小板时，开发的PDA脂质体-9 F9微阵列会产生强烈的荧光信号，并且还成功区分了在抗血小板模型药物替罗非班存在下血小板活化减少的情况。该单步台式试验的结果具有简单、灵敏和快速的属性，可以在所需的临床程序之前检测血小板活化的程度。
The necessity of a simple measurement of platelet activation has been increasing in clinical medicine to regulate the proper dose of the antiplatelet drugs for patients having clinical outcomes in acute situations such as angina pectoris, stroke, or peripheral vascular disease or procedures involving angioplasty or coronary thrombolysis. We developed a self-signaling polydiacetylene (PDA) liposome microarray to detect activated platelets from whole blood samples in a single step. A specific antibody, 9F9 antibody, to platelet-bound fibrinogen was selected and conjugated to the PDA liposome microarray to quantify the fibrinogen-bound platelets. The developed PDA liposome-9F9 microarray generated an intense fluorescence signal when activated platelets in whole blood were introduced and also successfully distinguished the reduced platelet activation in the presence of Tirofiban, a model antiplatelet drug. The results of this single-step benchtop assay incorporates simple, sensitive, and rapid attributes that can detect the extent of platelet activation prior to needed clinical procedures.