Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L.
Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L.
复制标题
DOI：
10.1016/j.chemosphere.2003.10.024
复制
发表时间：
2004-02-01
期刊：
影响因子：
8.8
通讯作者：
Agradi, E
中科院分区：
文献类型：
--
作者：
Labra, M;Grassi, F;Agradi, E
研究重铬酸钾对甘蓝型油菜遗传和DNA甲基化的影响。植物扩增片段长度多态性（AFLP）和选择性扩增多态性位点（SAMPL）的测试显示，暴露于10-200毫克/升重铬酸钾的小植株的序列改变剂量相关的增加。在相似条件下，不同的植株暴露于铬，表现出不同的AFLP和SAMPL DNA图谱。这些观察结果表明，随机的DNA突变重铬酸钾的反应，并反对优先突变位点。还评估了响应于铬处理的DNA甲基化变化。应用了两种互补的方法：（i）免疫标记，使用针对5-甲基胞嘧啶的单克隆抗体;和（ii）甲基化敏感扩增多态性（MSAP）。免疫标记显示，在甘蓝型油菜L。基因组中，当植物与重铬酸钾处理。MSAP分析显示CCGG序列中广泛的甲基化变化，净结果是全基因组超甲基化。这些结果表明，在植物中的DNA的变化作为对铬暴露的反应，这种影响是剂量依赖性的。不同标记检测的DNA多态性支持使用这些工具进行环境毒理学调查和通过DNA分析评估植物中污染物浓度的有效性。(C)2003 Elsevier Ltd.保留所有权利。
This study evaluated genetic and DNA methylation alteration induced by potassium dichromate in Brassica napus L. plants. Amplified fragment length polymorphism (AFLP) and selective amplification of polymorphic loci (SAMPL) tests revealed dose-related increases in sequence alterations in plantlets exposed to 10-200 mg/l potassium dichromate. Individual plantlets exposed to chromium under similar conditions showed different AFLP and SAMPL DNA profiles. These observations suggest random DNA mutation in response to potassium dichromate and argue against preferential sites for mutation. DNA methylation changes in response to chromium treatment were also evaluated. Two complementary approaches were applied: (i) immunolabelling, using a monoclonal antibody against 5-methylcytosine; and (ii) methylation-sensitive amplified polymorphism (MSAP). Immunolabelling showed cytosine-hypermethylation in the Brassica napus L. genome when plants were treated with potassium dichromate. MSAP analysis showed extensive methylation changes in CCGG-sequences, with the net result being genome-wide hypermethylation. These results showed a clear DNA alteration in plants as a response to chromium exposure and the effect was dose-dependent. DNA polymorphism detected by different markers supports the effectiveness of the use of these tools for the investigation of environmental toxicology and for evaluating the concentration of pollutants by DNA analysis in plants. (C) 2003 Elsevier Ltd. All rights reserved.