ZmMKK1, a novel group A mitogen-activated protein kinase kinase gene in maize, conferred chilling stress tolerance and was involved in pathogen defense in transgenic tobacco
ZmMKK1, a novel group A mitogen-activated protein kinase kinase gene in maize, conferred chilling stress tolerance and was involved in pathogen defense in transgenic tobacco
复制标题
DOI：
10.1016/j.plantsci.2013.09.014
复制
发表时间：
2014-01-01
期刊：
影响因子：
5.2
通讯作者：
Li, Dequan
中科院分区：
文献类型：
--
作者：
Cai, Guohua;Wang, Guodong;Li, Dequan
作为重要的细胞内信号转导模块，丝裂原活化蛋白激酶(MAPK)级联通路参与了植物对各种环境胁迫和病原菌攻击的信号转导。丝裂原活化蛋白激酶在多种应激信号传递过程中充当MAPK级联信号的汇聚点。本研究从玉米(Zea Mays L)中分离到一个新的MAPKK基因ZmMKK1，属于A组MAPKK，并对其功能进行了研究。ZmMKK1主要定位于细胞质，其组成型激酶活性形式ZmMKK1DD定位于细胞质和细胞核。QRT-PCR分析发现，ZmMKK1的表达是由非生物和生物胁迫以及外源信号分子触发的。此外，过氧化氢(H_2O_2)和钙离子(Ca~(2+))在12℃诱导ZmMKK1在mRNA水平上的表达上调。与空载体对照相比，ZmMKK1在烟草中的异源表达增强了抗氧化酶活性，增加了渗透调节物质的积累，显著上调了ROS相关基因和胁迫响应基因的表达，从而提高了烟草对低温胁迫的耐受性。此外，ZmMKK1在生物营养性和坏死性病原菌诱导的反应中发挥着不同的功能。这些结果表明，ZmMKK1在植物的低温胁迫和病原菌防御中起着重要作用。(C)2013爱思唯尔爱尔兰有限公司。保留所有权利。
As an important intracellular signaling module, the mitogen-activated protein kinase (MAPK) cascades have been previously implicated in signal transduction during plants responsing to various environmental stresses as well as pathogen attack. The mitogen-activated protein kinase kinase acts as the convergent point of MAPK cascades during a variety of stress signaling. In this study, a novel MAPKK gene, ZmMKK1, in maize (Zea mays L) belonging to group A MAPKK was isolated and functionally characterized. ZmMKK1 was mainly localized in the cytoplasm and its constitutive kinase-active form ZmMKK1DD was localized in both cytoplasm and nucleus. QRT-PCR analysis uncovered that ZmMKK1 expression was triggered by abiotic and biotic stresses and exogenous signaling molecules. Moreover, hydrogen peroxide (H2O2) and Ca2+ mediated 12 degrees C-induced up-regulated expressing of ZmMKK1 at mRNA level. Ectopic expression of ZmMKK1 in tobacco (Nicotiana tabacum) conferred tolerance to chilling stress by higher antioxidant enzyme activities, more accumulation of osmoregulatory substances and more significantly up-expression of ROS-related and stress-responsive genes compared with empty vector control plants. Furthermore, ZmMKK1 played differential functions in biotrophic versus necrotrophic pathogen-induced responses. These results suggested ZmMKK1 played a crucial role in chilling stress and pathogen defense in plants. (C) 2013 Elsevier Ireland Ltd. All rights reserved.