Self-assembly of quantum dots/denatured BSA-oligonucleotides bioconjugate and its application on aptameric gold nanoparticles-based biosensor for the determination of rHuEPO-α.
Self-assembly of quantum dots/denatured BSA-oligonucleotides bioconjugate and its application on aptameric gold nanoparticles-based biosensor for the determination of rHuEPO-α.
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DOI：
10.1016/j.bios.2012.12.040
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发表时间：
2013-05
影响因子：
12.6
通讯作者：
Jiefang Sun;Lei Guo;Hua Xu;Jijun Tang;Jianwei Xie
中科院分区：
文献类型：
--
作者：
Jiefang Sun;Lei Guo;Hua Xu;Jijun Tang;Jianwei Xie
本文以变性牛血清白蛋白（dBSA）为载体，通过与CdTe量子点表面金属配位位点的络合作用，将dBSA-ODNs共价杂化物自组装到CdTe量子点表面。所形成的量子点/dBSA-ODNs生物偶联物在水溶液中能保持长期稳定性，同时保持其荧光量子产率和光谱特性。此外，量子点/dBSA-ODNs还可以与抗rHuEPO-α适配体连接的金纳米粒子（Apt-AuNPs）杂交，形成基于荧光共振能量转移的纳米生物传感系统，以快速、简单的方式高灵敏、高选择性地检测浓度降至53.6pM的rHuEPO-α蛋白。
In this paper, denatured bovine serum albumin (dBSA) has been developed as a useful scaffold for appending multiple oligonucleotides (ODNs), and the resulted novel dBSA-ODNs covalent hybrid can be self-assembled to the surface of CdTe quantum dots (QDs) via complexation with unoccupied coordination metal sites on QDs' surface. The formed QDs/dBSA-ODNs bioconjugate could keep a long-term stability in aqueous solution while maintaining its fluorescence quantum yield and spectral properties. Furthermore, the QDs/dBSA-ODNs also can be readily hybridized with an anti-rHuEPO-α aptamers linked AuNPs (Apt-AuNPs) to form a fluorescent resonance energy transfer-based nano-biosensing system for highly sensitive and selective detection of rHuEPO-α protein lowered to 53.6pM in a rapid, simple manner.