Preliminary crystallographic studies of the amino terminal half of human lactoferrin in its iron-saturated and iron-free forms.
Preliminary crystallographic studies of the amino terminal half of human lactoferrin in its iron-saturated and iron-free forms.
复制标题
铁饱和和无铁形式的人乳铁蛋白氨基末端一半的初步晶体学研究。
DOI：
10.1016/0022-2836(92)90880-s
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发表时间：
1992
影响因子：
5.6
通讯作者：
Baker,EN
中科院分区：
文献类型：
--
作者：
Day,CL;Norris,GE;Anderson,BF;Tweedie,JW;Baker,EN
从转染的幼仓鼠肾细胞产生的人乳铁蛋白（Lf N）的氨基末端的一半已结晶在其铁饱和和无铁的形式。糖基化和去糖基化的Lf N晶体均为单斜晶系，空间群C2，晶胞尺寸a= 133· 0 A，B= 58· 3 A，c= 58·3 A，α= 90· 0°，β= 114· 7°，γ= 90· 0°，每个不对称单元一个分子。也已经使用去糖基化蛋白制备了apo Lf N的晶体。这些晶体为四元晶系，空间群为P4 1 2 1 2（或P4 3 2 1 2），晶胞尺寸为a= B= 58· 4 A/cm，c= 217· 2 A/cm，每个不对称单元一个分子。铁饱和晶体和无铁晶体都适用于高分辨率X射线分析。
The amino terminal half of human lactoferrin (Lf N) produced from transfected baby hamster kidney cells has been crystallized in its iron-saturated and iron-free forms. The crystals of glycosylated Lf N and deglycosylated Lf N are monoclinic, space group C2, with cell dimensions a= 133· 0 A ̊, b= 58· 3 A ̊, c= 58· 3 A ̊, α= 90· 0°, β= 114· 7°, γ= 90· 0°, and one molecule per asymmetric unit. Crystals of apo Lf N have also been prepared using deglycosylated protein. These crystals are tetragonal, space group P4 1 2 1 2 (or P4 3 2 1 2), with cell dimensions of a= b= 58· 4 A ̊ and c= 217· 2 A ̊ and one molecule per asymmetric unit. Both the iron-saturated and the iron-free crystals are suitable for high resolution X-ray analysis.