Regulating infidelity: RNA-mediated recruitment of AID to DNA during class switch recombination.
Regulating infidelity: RNA-mediated recruitment of AID to DNA during class switch recombination.
复制标题
调节不忠：类别转换重组过程中 RNA 介导的 AID 向 DNA 的招募。
DOI：
10.1002/eji.201545809
复制
发表时间：
2016
影响因子：
5.4
通讯作者：
Chaudhuri,Jayanta
中科院分区：
文献类型：
--
作者：
DiMenna,LaurenJ;Chaudhuri,Jayanta
DNA脱氨酶激活诱导的胞苷脱氨酶（AID）在类别转换重组期间特异性募集到重复转换区DNA的机制仍然知之甚少。过去十年的工作揭示了AID招募中转录和RNA聚合酶相关因素之间的密切联系，但这些过程都没有令人满意地解释AID特异性如何受到影响。在这里，我们回顾了最近的一项发现，其中AID被引导开关区域不是由蛋白质因子，而是由RNA部分，特别是与非编码RNA，一直被认为是惰性的。这项工作解释了在类别转换过程中非编码转录本剪接的长期需求，并对B细胞介导的免疫以及与重组反应相关的潜在病理综合征都有影响。
The mechanism by which the DNA deaminase activation‐induced cytidine deaminase (AID) is specifically recruited to repetitive switch region DNA during class switch recombination is still poorly understood. Work over the past decade has revealed a strong link between transcription and RNA polymerase‐associated factors in AID recruitment, yet none of these processes satisfactorily explain how AID specificity is affected. Here, we review a recent finding wherein AID is guided to switch regions not by a protein factor but by an RNA moiety, and especially one associated with a noncoding RNA that has been long thought of as being inert. This work explains the long‐standing requirement of splicing of noncoding transcripts during class switching, and has implications in both B cell‐mediated immunity as well as the underlying pathological syndromes associated with the recombination reaction.