A Study of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Induced Liver Injury in Jian Carp (Cyprinuscarpio var. Jian) Using Precision-Cut Liver Slices
A Study of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Induced Liver Injury in Jian Carp (Cyprinuscarpio var. Jian) Using Precision-Cut Liver Slices
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DOI：
10.1007/s00128-015-1683-5
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发表时间：
2015
影响因子：
2.7
通讯作者：
Jin-liang Du;Li-ping Cao;Yingjuan Liu;Rui Jia;Guo-Jun Yin
中科院分区：
文献类型：
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作者：
Jin-liang Du;Li-ping Cao;Yingjuan Liu;Rui Jia;Guo-Jun Yin
本研究的目的是利用精密切割肝切片（PCLS）建立用于研究2,3,7,8-四氯二苯并-对二恶英（TCDD）引起的建鲤肝损伤的模型。用浓度为 0、0.05、0.1、0.3 和 0.6 μg/L 的 TCDD 处理 PCLS 6 小时，然后收集培养物上清液和 PCLS 进行分析。分析了几种生化指标，包括谷氨酸丙酮酸转氨酶（GPT）、谷氨酸草酰乙酸转氨酶（GOT）、乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）。还评估了细胞色素 P4501A (CYP1A)、芳烃受体 2 (AhR2) 和芳烃受体核转位子 2 (ARNT2) 的 mRNA 表达。结果表明，在 TCDD 浓度低至 0.05 µg/L 时，观察到对 MDA、GSH-Px 和 PCLS 活力的一些显着影响 (p< 0.05)，并且观察到的影响随着暴露浓度的增加而增加。暴露于浓度为 0.3 μg/L 的 TCDD 6 小时后，观察到 GPT、GOT、MDA 和 LDH 含量显着增加（p<0.01），而 SOD 活性、GSH-Px 活性和 PCLS 活力降低（p<0.01 或 p<0.05）。暴露于 0.3 µg/L TCDD 也会导致 CYP1A、AhR2 和 ARNT2 的 mRNA 表达增加。总体而言，这些结果提供了 TCDD 诱导建鲤肝损伤和氧化应激的证据。这些结果也支持使用PCLS作为评价建鲤肝毒性的体外模型。
The aim of this study was to establish a model for the study of liver injury induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Jian carp using precision-cut liver slices (PCLS). PCLS were treated with TCDD at concentrations of 0, 0.05, 0.1, 0.3, and 0.6 μg/L for 6 h, followed by collection of the culture supernatant and PCLS for analysis. Several biochemical indices were analyzed, including glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA). Expression of mRNA was also estimated for cytochrome P4501A (CYP1A), aryl hydrocarbon receptor2 (AhR2), and aryl hydrocarbon receptor nuclear translocator2 (ARNT2). Results showed that some significant effects (p< 0.05) in MDA, GSH-Px and PCLS viability were observed at a TCDD concentration as low as 0.05 µg/L, and the observed effects increased with exposure concentration. Following exposure to TCDD for 6 h at a concentration of 0.3 μg/L, significant increases (p< 0.01) in the content of GPT, GOT, MDA, and LDH were observed, while SOD activity, GSH-Px activity, and PCLS viability were decreased (p< 0.01 orp< 0.05). Exposure to 0.3 μg/L TCDD also resulted in increased expression of mRNA for CYP1A, AhR2, and ARNT2. Overall, these results provide evidence of TCDD-induced liver injury and oxidative stress in Jian carp. These results also support the use of PCLS as an in vitro model for the evaluation of hepatotoxicity in Jian carp.