Characterization of a 1200-kb genomic segment of chromosome 3p22-p21.3.
Characterization of a 1200-kb genomic segment of chromosome 3p22-p21.3.
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DOI：
10.1093/dnares/6.1.37
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发表时间：
1999-02-26
期刊：
影响因子：
--
通讯作者：
Nakamura, Y
中科院分区：
文献类型：
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作者：
Daigo, Y;Isomura, M;Nakamura, Y
我们先前确定的核苷酸序列和特征的685 kb的近端一半的CEPH YAC 936 c1，这对应于人类染色体3p21.3的一部分。在这里报道的研究中，我们的特点是剩余的515 kb的YAC克隆对应于其人类插入端粒的一半。新测序的区域共包含10个基因，包括6个先前报道的基因：磷脂酶C δ 1（PLCD 1），人激活素受体IIB型（hActR-IIB），有机阳离子转运蛋白样1（OCTL 1），有机阳离子转运蛋白样2（OCTL 2），氧化应激反应1（OSR 1）和人木酮糖激酶样蛋白（XYLB）。端粒区域中存在的其余四个基因包括两个已知基因MyD 88和ACAA，以及两个新基因。发现其中一个新序列（命名为ENGL）编码与DNA/RNA核酸内切酶家族，特别是核酸内切酶G同源的氨基酸序列。另一个基因F56与任何已知基因均无明显同源性。这些结果揭示了YAC 936 c1中存在的3 p的1200-kb区域的完整物理和转录图谱。
We previously determined the nucleotide sequence and characterized the 685-kb proximal half of CEPH YAC936c1, which corresponds to a portion of human chromosome 3p21.3. In the study reported here, we characterized the remaining 515-kb of this YAC clone corresponding to the telomeric half of its human insert. The newly sequenced region contained a total of ten genes including six reported previously: phospholipase C delta 1 (PLCD1), human activin receptor type IIB (hActR-IIB), organic cation transporter-like 1 (OCTL1), organic cation transporter-like 2 (OCTL2), oxidative stress response 1 (OSR1), and human xylulokinase-like protein (XYLB). The remaining four genes present in the telomeric region included two known genes, MyD88 and ACAA, and two novel genes. One (designated ENGL) of the novel sequences was found to encode an amino-acid sequence homologous to the family of DNA/RNA endonucleases, especially endonuclease G. The other gene F56 revealed no significant homology to any known genes. These results disclosed complete physical and transcriptional maps of the 1200-kb region of 3p present in YAC 936c1.