Cell-cell transmission allows human T-lymphotropic virus 1 to circumvent tetherin restriction
Cell-cell transmission allows human T-lymphotropic virus 1 to circumvent tetherin restriction
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DOI：
10.1016/j.virol.2012.11.012
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发表时间：
2013-02-05
期刊：
影响因子：
3.7
通讯作者：
Heidecker, Gisela
中科院分区：
文献类型：
--
作者：
Ilinskaya, Anna;Derse, David;Heidecker, Gisela
Tetherin是细胞先天免疫的一部分，通过将病毒留在细胞表面来阻止从质膜萌发的病毒的无细胞传播。一些病毒在VPU(HIV-1)、K-蛋白(KSHV)、Nef(SIV)或Env(HIV-2)等不同的蛋白质中进化出活性来下调Tetherin并克服其限制。我们发现，长期感染HTLV-1的T细胞系比未感染的转化T细胞系表达的Tetherin多8倍，这表明Tetherin的表达不受病毒的抑制。我们观察到，即使是少量的外源性Tetherin也会导致HTLV-1滞留在细胞表面，并严重降低HTLV-1的无细胞感染性，但与HTLV-1更相关的细胞间传播性下降明显较少。然而，敲除Tetherin表达导致细胞-细胞感染略有增加，这表明该蛋白不会增强这一传播途径。由爱思唯尔公司出版。
Tetherin is part of the cellular innate immunity and impedes cell-free transmission of viruses that bud from the plasma membrane by retaining them on the cell surface. Some viruses have evolved activities in different proteins such as Vpu (HIV-1), K-protein (KSHV), Nef (SIV) or Env (HIV-2) to downregulate tetherin and overcome its restriction. We found that chronically HTLV-1 infected T-cell lines express eightfold more tetherin than uninfected transformed T-cell lines suggesting that tetherin expression is not inhibited by the virus. We observed that even small amounts of exogenous tetherin caused the retention of HTLV-1 on the cell surface and severely reduced cell-free infectivity of HTLV-1, but that cell-cell transmission, which is more relevant for HTLV-1, was significantly less decreased. However, knock-down of tetherin expresssion resulted in a slight increase in cell-cell infection indicating that the protein does not enhance this route of transmission. Published by Elsevier Inc.