Detection of pesticide residues using an immunodevice based on negative dielectrophoresis
Detection of pesticide residues using an immunodevice based on negative dielectrophoresis
复制标题
DOI：
10.1016/j.bios.2008.08.035
复制
发表时间：
2009-02-15
影响因子：
12.6
通讯作者：
Matsue, Tomokazu
中科院分区：
文献类型：
--
作者：
Ramon-Azcon, Javier;Kunikata, Ryouta;Matsue, Tomokazu
介绍了一种基于负介电泳（n-DEP）的微流控光学免疫传感技术对阿特拉津的检测。阿特拉津是一种毒性大的三嗪类除草剂，在除草剂中使用最频繁。聚苯乙烯微粒（6 μ m直径）修饰牛血清白蛋白与莠去津（莠去津-BSA）进行了操纵和捕获时，受到强烈的n-DEP电场。具体地，当向电极施加振幅为10 V峰值且频率超过1 MHz的AC电压时，捕获颗粒。用于检测阿特拉津的颗粒上发生的免疫反应是基于使用用异硫氰酸荧光素标记的第二抗小鼠免疫球蛋白G（IgC）抗体的间接竞争测定。微流体装置，与三维微电极，制造包括两个笼状区域，允许两个同时测量同一微流体通道内。使用葡萄酒样本评估了这种n-DEP免疫传感技术的性能。免疫装置显示阿特拉津的检测限缓冲液样品中的0.11微克L-1和预处理的葡萄酒样品中的6.8微克L-1，这些检测限低于最大残留量（MRL）由欧洲共同体建立的这种除草剂在葡萄酒中的残留物（50微克L-1）。这种方法为快速，简单，成本效益高，现场分析生物，食品和饮料，以及环境样品提供了很大的希望。(C)2008 Elsevier B. V.保留所有权利。
The detection of atrazine using a novel optical immunosensing technique based on negative dielectrophoresis (n-DEP) in microfluidic channels is described. Atrazine is a toxic triazine herbicide within the most frequently used. Polystyrene microparticles (6 mu m diameters) modified with bovine serum albumin conjugated with atrazine (atrazine-BSA) were manipulated and captured when subjected to intense n-DEP electric fields. Specifically, particles were trapped when AC voltages with amplitudes of 10 V-peak and frequencies over 1 MHz were applied to the electrodes. The immunological reaction occurring on the particles for detecting atrazine is based on an indirect competitive assay using a secondary anti-mouse immunogloburin G (IgC) antibody labeled with fluorescein isothiocyanate. The microfluidic device, with three-dimensional microelectrodes, was fabricated comprising two caged areas, allowing two simultaneous measurements inside the same microfluidic channel. The performance of this n-DEP immunosensing technique was evaluated using wine samples. The immunodevice showed a limit of detection for atrazine in buffer samples of 0.11 mu g L-1 and in pre-treated wine samples of 6.8 mu g L-1; these detection limits are lower than the maximum residue level (MRL) established by the European Community for residues of this herbicide in wine (50 mu g L-1). This methodology offers great promise for rapid, simple, cost effective, and on-site analysis of biological, foods and beverages, and environmental samples. (C) 2008 Elsevier B.V. All rights reserved.