Determination of calcium, sodium, potassium and magnesium concentrations in human senile cataractous lenses
Determination of calcium, sodium, potassium and magnesium concentrations in human senile cataractous lenses
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DOI：
10.1002/1099-0844(200012)18:4
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发表时间：
2000-12-01
影响因子：
3.6
通讯作者：
Atas, M
中科院分区：
文献类型：
--
作者：
Dilsiz, N;Olcucu, A;Atas, M
已经发现白内障晶状体具有细胞内离子环境的分布，相对于大多数细胞的胞质溶胶，钾和镁的浓度降低，而钠和钙的浓度增加。这是由于透镜膜特性的变化导致透镜膜渗透性增加的结果。已经发现这些变化是由于人类透镜的正常老化而引发的。本文用原子吸收光谱法和火焰发射光谱法测定了正常人晶状体和白内障晶状体中Ca（2+）、K（+）、Na（+）和Mg（2+）的含量。正常人透镜Ca（2+）在0.15和0.5 μ mol g（-1）新鲜透镜重量之间;在老年性白内障中，该值增加到9.31 μ mol g（-1）（p < 0.0001）。Na（+）、Mg（2+）和K（+）的正常水平分别为20、5.5和60 μ mol g（-1），在老年性白内障患者晶状体中分别为136.10、3.60和9.33 μ mol g（-1）（p < 0.002、p < 0.002和p < 0.01）。这些元素的显著差异可能在白内障的发生中起一定作用。版权所有（C）2000约翰威利父子有限公司
Cataractous lenses have been found to have a distribution of the intracellular ionic environment, the concentrations of potassium and magnesium decreasing and the concentrations of sodium and calcium increasing relative to the cytosol of most cells. This arises as a result of changes to lens membrane characteristics causing an increase in lens membrane permeability. These changes have been found to be initiated as a result of normal ageing of the human lens. In this study, total Ca(2+), K(+), Na(+) and Mg(2+) contents have been determined in human normal and cataractous lenses using atomic absorption and flame emission spectroscopy. The normal human lens Ca(2+) is between 0.15 and 0.5 mu mol g(-1) fresh lens weight; in senile cataracts the value increased up to 9.31 mu mol g(-1) (p < 0.0001). The normal levels of Na(+), Mg(2+) and K(+) are 20, 5.5 and 60 mol g(-1) respectively; these changed to 136.10, 3.60 and 9.33 mu mol g(-1), respectively in cataractous senile human lenses (p < 0.002, p < 0.002 and p < 0.01). The remarkable differences in these elements may play some role in cataractogenesis. Copyright (C) 2000 John Wiley & Sons, Ltd.