The OhrR repressor senses organic hydroperoxides by reversible formation of a cysteine-sulfenic acid derivative
The OhrR repressor senses organic hydroperoxides by reversible formation of a cysteine-sulfenic acid derivative
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DOI：
10.1073/pnas.102483199
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发表时间：
2002-05-14
影响因子：
11.1
通讯作者：
Helmann, JD
中科院分区：
文献类型：
--
作者：
Fuangthong, M;Helmann, JD
活性氧能诱导在有氧环境中对生命至关重要的解毒和修复基因的表达。感受到活性氧的细菌因子使用硫代二硫键交换反应(OxyR，RsrA)或氧化还原不稳定的2Fe-2S簇(SoxR)。我们证明了枯草芽孢杆菌OhrR的简化形式与OhrA控制区中两个相邻的反向重复序列协同结合，从而抑制转录。在有机过氧化氢存在下，OhrR被一个保守的半胱氨酸残基可逆地氧化为相应的半胱氨酸-磺酸，并可能被氧化到更高的氧化态而失活。
Reactive oxygen species induce the expression of detoxification and repair genes critical for life in an aerobic environment. Bacterial factors that sense reactive oxygen species use either thioldisulfide exchange reactions (OxyR, RsrA) or redox labile 2Fe-2S clusters (SoxR). We demonstrate that the reduced form of Bacillus subtilis OhrR binds cooperatively to two adjacent inverted repeat sequences in the ohrA control region and thereby represses transcription. In the presence of organic hydroperoxides, OhrR is inactivated by the reversible oxidation of a single conserved cysteine residue to the corresponding cysteine-sulfenic acid, and perhaps to higher oxidation states.