Analysis of Follicle-Stimulating Hormone Receptor in Infantile Hemangioma.
Analysis of Follicle-Stimulating Hormone Receptor in Infantile Hemangioma.
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DOI：
10.1097/sap.0000000000001438
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发表时间：
2018-04
影响因子：
1.5
通讯作者：
Greene AK
中科院分区：
文献类型：
--
作者：
Maclellan RA;Konczyk DJ;Goss JA;Greene AK
婴儿血管瘤（IH）的生命周期和卵泡刺激素（FSH）的分泌是相同的。我们之前已经证明IH含有促卵泡激素（FSHR）受体。本研究的目的是确定IH中哪种细胞类型表达FSHR。在临床指示的外科手术过程中获得的人类增殖IH组织被使用。石蜡切片和分离的细胞群（内皮细胞、周细胞、干细胞）进行免疫荧光检测FSHR。用DAPI、抗α平滑肌肌动蛋白或生物素化ULEX Europaeus凝集素I对组织进行共染色，分别鉴定细胞核、周细胞和内皮细胞。整个组织和纯化的单细胞群体采用PCR检测FSHR。阳性对照标本（卵巢、支持细胞）和阴性对照组织（皮肤/皮下、肝细胞）。9个IHs的免疫荧光显示，与内皮细胞相比，FSHR在周细胞中富集。FSHR在6个全组织his中有6个与PCR阳性对照一起表达。阴性对照样品中不存在FSHR。5组周细胞中有4组通过PCR表达FSHR。通过PCR检测，IH内皮细胞、IH干细胞和阴性对照细胞均未表现出FSHR。由于FSH的分泌与IH的生长模式相关，因此FSH可能参与了疾病过程。FSHR在IH的周细胞中富集，表明这种细胞类型可能参与了肿瘤的发病机制。
The life-cycle of infantile hemangioma (IH) and secretion of follicle-stimulating hormone (FSH) are identical. We previously have shown that IH contains the receptor for follicle-stimulating hormone (FSHR). The purpose of this study was to identify which cell type(s) in IH expresses FSHR. Human proliferating IH tissues obtained during a clinically-indicated surgical procedure were used. Paraffin sections and isolated cell populations (endothelial, pericyte, stem cell) were subjected to immunofluorescence for FSHR. Tissues were co-stained with DAPI, anti-α smooth muscle actin, or biotinylated ULEX Europaeus Agglutinin I to identify nuclei, pericytes, and endothelial cells, respectively. Whole tissue and purified single cell populations underwent PCR for FSHR. Positive control specimens (ovary, sertoli cells) and negative control tissues (skin/subcutis, hepatic cells) were included. Immunofluorescence of 9 IHs demonstrated that FSHR was enriched in pericytes compared to endothelial cells. FSHR was expressed in 6 of 6 whole tissue IHs along with the positive control via PCR. FSHR was not present in the negative control samples. Four of 5 sets of pericytes expressed FSHR by PCR. Neither IH endothelial cells, IH stem cells, or negative control cells exhibited FSHR by PCR. Because the secretion of FSH correlates with the growth pattern of IH, FSH might be involved in the disease process. FSHR is enriched in the pericytes of IH suggesting that this cell type may be involved in the pathogenesis of the tumor.