Polaprezinc down-regulates proinflammatory cytokine-induced nuclear factor-kappaB activiation and interleukin-8 expression in gastric epithelial cells.
Polaprezinc down-regulates proinflammatory cytokine-induced nuclear factor-kappaB activiation and interleukin-8 expression in gastric epithelial cells.
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Polaprezinc 下调胃上皮细胞中促炎细胞因子诱导的核因子 kappaB 激活和白细胞介素 8 表达。
DOI：
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发表时间：
1999
影响因子：
3.5
通讯作者：
A. Terano
中科院分区：
文献类型：
--
作者：
T. Shimada;N. Watanabe;Y. Ohtsuka;M. Endoh;K. Kojima;H. Hiraishi;A. Terano
胃上皮细胞趋化因子反应是幽门螺杆菌感染相关的胃炎症诱导的主要因素。由于持续的炎症是胃粘膜损伤的风险，因此下调炎症反应的药物可能具有治疗意义。我们研究了Polaprezinc（一种有效的抗溃疡剂）对胃上皮细胞中促炎性白细胞介素（IL）-8表达的影响。由于IL-8的表达受转录因子核因子-κ B（NF-κ B）的调节，我们还研究了polaprezinc对NF-κ B活性的影响。使用MKN 28细胞作为胃上皮细胞的模型。采用IL-8特异性酶联免疫吸附测定法测定IL-8的分泌量，采用北方印迹法检测IL-8 mRNA的表达。通过电泳迁移率变动分析来分析NF-κ B活性。用抗磷酸化IkappaB-α抗体进行蛋白质印迹分析以评估IkappaB-α磷酸化。Polaprezin以剂量依赖性方式抑制肿瘤坏死因子α（TNF-α）或IL-1 β诱导的IL-8分泌。Polaprezinc也抑制IL-8 mRNA的表达。Polaprezinc可下调TNF-α、IL-1 β、佛波酯和H2O引起的NF-κ B活化。蛋白质印迹分析显示，在polaprezinc存在下，TNF-α诱导的IkappaB-α磷酸化受到抑制。总的来说，这些结果表明，polaprezinc是一种新型的抗炎剂，下调胃粘膜细胞的炎症反应。
Gastric epithelial chemokine response is a primary factor in the induction of gastric inflammation associated with Helicobacter pylori infection. Because sustained inflammation is a risk for gastric mucosal damage, agents that down-regulate inflammatory responses may be of therapeutic significance. We examined the effect of polaprezinc, a potent antiulcer agent, on proinflammatory cytokine-induced interleukin (IL)-8 expression in gastric epithelial cells. Because IL-8 expression is regulated by the transcription factor nuclear factor-kappaB (NF-kappaB), we also examined the effect of polaprezinc on NF-kappaB activity. MKN28 cells were used as a model of gastric epithelial cells. Secreted IL-8 was quantified by IL-8 specific enzyme-linked immunosorbent assay, and IL-8 mRNA expression was examined by Northern blot analysis. NF-kappaB activity was analyzed by electrophoretic mobility shift assay. Western blot analysis with anti-phospho-IkappaB-alpha antibody was performed to assess IkappaB-alpha phosphorylation. Polaprezinc-suppressed IL-8 secretion induced by tumor necrosis factor alpha (TNF-alpha) or IL-1beta in a dose-dependent manner. IL-8 mRNA expression also was inhibited by polaprezinc. NF-kappaB activation in response to TNF-alpha, IL-1beta, phorbol ester, and H(2)O(2) was down-regulated by polaprezinc. Western blot analysis showed inhibition of TNF-alpha-induced IkappaB-alpha phosphorylation in the presence of polaprezinc. Collectively, these results suggest that polaprezinc is a novel type of anti-inflammatory agent that down-regulates inflammatory responses of gastric mucosal cells.
影响因子：
29.4
作者：
Keates, S;Hitti, YS;Kelly, CP
通讯作者：
Kelly, CP
影响因子：
1.8
作者：
Atta, Ananta Kumar;Ahn, In-Ho;Cho, Dong-Gyu
通讯作者：
Cho, Dong-Gyu
DOI：
10.1080/07315724.1997.10718706
发表时间：
1997-10-01
影响因子：
3.5
作者：
Connell, P;Young, VM;Hennig, B
通讯作者：
Hennig, B