Interrelationships between mitochondrial fusion, energy metabolism and oxidative stress during development in Caenorhabditis elegans.
Interrelationships between mitochondrial fusion, energy metabolism and oxidative stress during development in Caenorhabditis elegans.
复制标题
DOI：
10.1016/j.bbrc.2010.12.017
复制
发表时间：
2011-01
影响因子：
3.1
通讯作者：
K. Yasuda;P. Hartman;Takamasa Ishii;H. Suda;A. Akatsuka;T. Shoyama;Masaki Miyazawa;N. Ishii
中科院分区：
文献类型：
--
作者：
K. Yasuda;P. Hartman;Takamasa Ishii;H. Suda;A. Akatsuka;T. Shoyama;Masaki Miyazawa;N. Ishii
已知线粒体是动态结构，具有能量和酶介导的融合和分裂过程，负责维持恒定的通量。线粒体还作为能量代谢的副产品在活性氧产生中发挥作用。在目前的研究中，线粒体融合，能量代谢和氧化应激对发展之间的相互关系进行了探讨，使用fzo-1突变体的融合过程中的缺陷和mev-1突变体过量生产超氧化物从线粒体电子传递复合体II的秀丽隐杆线虫。虽然两个单突变体的生长和发育相对于野生型略有延迟，但fzo-1;mev-1双突变体经历了相当大的延迟。在幼虫发育过程中的氧敏感性，超氧阴离子的产生和羰基蛋白的积累fzo-1突变体与野生型相似。fzo-1动物的代谢显著低于N2和mev-1。这些数据表明，线粒体融合可以深刻地影响能量代谢和发育。
Mitochondria are known to be dynamic structures with the energetically and enzymatically mediated processes of fusion and fission responsible for maintaining a constant flux. Mitochondria also play a role of reactive oxygen species production as a byproduct of energy metabolism. In the current study, interrelationships between mitochondrial fusion, energy metabolism and oxidative stress on development were explored using a fzo-1 mutant defective in the fusion process and a mev-1 mutant overproducing superoxide from mitochondrial electron transport complex II of Caenorhabditis elegans. While growth and development of both single mutants was slightly delayed relative to the wild type, the fzo-1;mev-1 double mutant experienced considerable delay. Oxygen sensitivity during larval development, superoxide production and carbonyl protein accumulation of the fzo-1 mutant were similar to wild type. fzo-1 animals had significantly lower metabolism than did N2 and mev-1. These data indicate that mitochondrial fusion can profoundly affect energy metabolism and development.