Purification and reconstitution of a 75-kilodalton protein identified as a component of the renal Na+/glucose symporter.
Purification and reconstitution of a 75-kilodalton protein identified as a component of the renal Na+/glucose symporter.
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纯化和重建 75 千道尔顿蛋白质，该蛋白质被鉴定为肾钠 / 葡萄糖同向转运蛋白的组成部分。
DOI：
10.1021/bi00393a002
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Lever,JE
中科院分区：
文献类型：
--
作者：
Wu,JS;Lever,JE
这些观察使我们提出了将主链定向分配策略应用于蛋白质2D ′ HNMR谱分析的下列算法。(1)NH-C ^ H单元：通过分析N-相关光谱定义所有明显的NH-CaH-C^ H N-偶联组。(2)受阻：（a）搜索两个连续互锁螺旋MCD模式的所有组;（B）在两个方向上延伸这些组中的每一个;（c）从进一步的双链化中去除仅涉及这些残基的主链质子的所有NOE。(3)反平行片层：（a）寻找完整的反平行片层模式;（B）通过应用杂合物和（如果存在）额外的完整反平行片层模式来扩展每个完整模式;（c）从进一步考虑中删除仅涉及这些残基的主链质子的所有NOE。(4)平行片：（a）寻找双平行片模式;（B）通过应用单平行片模式来扩展每个双平行片模式;（c）从进一步的考虑中排除所有只涉及这些残基的主链质子的NOE。(5)将（3）和（4）的结果进行比较。构造找到的MCD定义的表并合并（2）和（3）揭示的转弯。
These observations lead us to propose the following algo-rithm for applying the main-chain-directed assignment strategy to the analysis of 2D'Hnmr spectra of proteins.(1) NH-^ HC^ H units: define all apparent NH-CaH-C^ H/-coupled sets through analysis of/-correlated spectra.(2) Helix:(a) search for all groups of two consecutive interlocking helical MCD patterns;(b) extend each of these groups in both directions;(c) remove from further consider-ation all NOEs exclusively involving the main-chain protons of these residues.(3) Antiparallel sheet:(a) search for the full antiparallel sheet pattern;(b) extendeach full pattern by application of the hybrid and, when present, additional full antiparallel sheet patterns;(c) remove from further consideration all NOEs exclusively involving the main-chain protons of these residues.(4) Parallel sheet:(a) search for the double parallel sheet pattern;(b) extendeach doubleparallel sheet pattern byap-plication of thesingle parallel sheet pattern;(c) remove from further consideration all NOEsexclusively involving the main-chain protons of these residues.(5) Reconcile the results of (3) and (4). Construct the MCD-defined sheets found and incorporate turns revealed by (2) and (3).