Identification and disruption of the gene encoding the third member of the low-molecular-mass rhoptry complex in Plasmodium falciparum
Identification and disruption of the gene encoding the third member of the low-molecular-mass rhoptry complex in Plasmodium falciparum
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DOI：
10.1128/iai.70.9.5236-5245.2002
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发表时间：
2002-09-01
影响因子：
3.1
通讯作者：
Cowman, AF
中科院分区：
文献类型：
--
作者：
Baldi, DL;Good, R;Cowman, AF
恶性疟原虫的低分子量棒状体复合物由棒状体相关蛋白1（RAPT）、RAP 2和RAP 3三种蛋白质组成。编码RAPT和RAP 2的基因是已知的;然而，RAP 3基因尚未被鉴定。在这项研究中，我们从恶性疟原虫基因组数据库中鉴定了RAP 3基因，并表明该蛋白质是低分子量棒状体复合物的一部分。RAP 3的破坏表明它不是裂殖子入侵所必需的，可能是因为RAP 2可以补充RAP 3的丢失。RAP 3与RAP 2具有同源性，并且基因以头对尾的方式编码在5号染色体上。对基因组数据库的分析已经在所有疟原虫属中鉴定出同源基因，提示该蛋白在裂殖子侵入中起作用。约氏疟原虫基因组中RAP 3同源物周围的区域与恶性疟原虫中的相同区域同线;然而，存在单个基因。疟原虫RAP 2/3蛋白家族的系统发育比较表明RAP 2/3复制发生在这些寄生虫种分化之后。
The low-molecular-mass rhoptry complex of Plasmodium falciparum consists of three proteins, rhoptry-associated protein 1 (RAPT), RAP2, and RAP3. The genes encoding RAPT and RAP2 are known; however, the RAP3 gene has not been identified. In this study we identify the RAP3 gene from the P. falciparum genome database and show that this protein is part of the low-molecular-mass rhoptry complex. Disruption of RAP3 demonstrated that it is not essential for merozoite invasion, probably because RAP2 can complement the loss of RAP3. RAP3 has homology with RAP2, and the genes are encoded on chromosome 5 in a head-to-tail fashion. Analysis of the genome databases has identified homologous genes in all Plasmodium spp., suggesting that this protein plays a role in merozoite invasion. The region surrounding the RAP3 homologue in the Plasmodium yoelii genome is syntenic with the same region in P. falciparum; however, there is a single gene. Phylogenetic comparison of the RAP2/3 protein family from Plasmodium spp. suggests that the RAP2/3 duplication occurred after divergence of these parasite species.