μ-Opioid-receptor-mediated inhibidon of the N-type calcium-channel current
μ-Opioid-receptor-mediated inhibidon of the N-type calcium-channel current
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μ-阿片受体介导的 N 型钙通道电流抑制
DOI：
--
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
G. Henderson
中科院分区：
文献类型：
--
作者：
E. Seward;C. Hammond;G. Henderson
P-阿片受体激活的主要后果是抑制神经元活性和递质释放。先前已经观察到μ-阿片样物质激动剂增加钾电导并抑制腺苷酸环化酶。我们现在报道，μ-阿片受体的激活直接降低了分化的人神经母细胞瘤细胞系（SHSY 5 Y）中的N型钙通道电流。μ-阿片受体与钙通道的偶联涉及百日咳毒素不敏感的G蛋白，并且不依赖于腺苷酸环化酶活性的变化。钙通道电流的抑制是电压依赖性的，因为它在很大程度上被强的膜去极化所克服。它与电流失活动力学的变化无关。因此，μ受体属于G蛋白偶联的抑制性神经递质受体超家族，其调节钙和钾通道以及腺苷酸环化酶的活性。
The predominant consequences of p-opioid-receptor activation are depression of both neuronal activity and transmitter release. μ-Opioid agonists have previously been observed to increase a potassium conductance and to inhibit adenylate cyclase. We now report that activation of μ-opioid receptors directly decreases the N-type calcium-channel current in a differentiated, human neuroblastoma cell line (SHSY5Y). The coupling between the μ-opioid receptor and the calcium channel involves a pertussis toxinsensitive G protein and is independent of changes in adenylate cyclase activity. The inhibition of the calcium-channel current is voltage dependent because it is largely overcome by strong membrane depolarization. It is not associated with changes in the kinetics of current inactivation. Therefore, the μ-receptor belongs to the superfamily of G-protein-coupled, inhibitory neurotransmitter receptors which modulate the activity of calcium and potassium channels and adenylate cyclase.
神经母细胞瘤-神经胶质瘤杂交 NG108-15 细胞中脑啡肽和生长抑素阻断钙通道。
DOI：
10.1073/pnas.83.24.9832
发表时间：
1986
影响因子：
11.1
作者：
Tsunoo,A;Yoshii,M;Narahashi,T
通讯作者：
Narahashi,T