Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage M1.
Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage M1.
复制标题
马铃薯黑胫病病原体胡萝卜软腐欧文氏菌亚种的普遍转导。
作者：
Ian K. Toth;V. Mulholland;V. Cooper;Stephen D. Bentley;Yu;M. Pérombelon;G. Salmond
利用富集方法，分离到一株能广泛转导胡萝卜软腐欧文氏菌（Erwinia carotovora subsp.）的噬菌体（M1）。萎缩败血性菌（Eca）菌株SCRI1043。M1可能是一种毒性噬菌体，含有约43 kb的双链DNA。建立了许多染色体标记和质粒pHCP 2的转导频率，并优化了转导条件。在转导之前对裂解物进行UV照射增强了转导频率。M1感染超过25%的Eca菌株测试，因此可能是有用的遗传分析的一些Eca分离株和菌株之间的选择标记的转导转移。
Using enrichment methods, a new bacteriophage (M1) was isolated, which is capable of generalized transduction in Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043. M1 is probably a virulent phage and contains double-stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2 were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. M1 infected over 25% of Eca strains tested and so may be useful both for the genetic analysis of a number of Eca isolates and for the transductional transfer of selectable markers between strains.