Docosahexaenoic acid intake ameliorates ketamine-induced impairment of spatial cognition and learning ability in ICR mice
Docosahexaenoic acid intake ameliorates ketamine-induced impairment of spatial cognition and learning ability in ICR mice
复制标题
二十二碳六烯酸摄入可改善氯胺酮引起的 ICR 小鼠空间认知和学习能力损伤
DOI：
10.1016/j.neulet.2014.08.011
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发表时间：
2014-09-19
影响因子：
2.5
通讯作者：
Chen, Hongxian
中科院分区：
文献类型：
--
作者：
Huang, Shucai;Dai, Yuanyuan;Chen, Hongxian
几项研究报告了氯胺酮引起的认知障碍。补充二十二碳六烯酸 (DHA) 可改善人类婴儿的认知功能，并预防阿尔茨海默病 (AD) 患者的学习障碍。在这项研究中，我们研究了 DMA 对氯胺酮引起的癌症研究所 (ICR) 小鼠空间认知和学习能力损害的影响。莫里斯水迷宫（MWM）用于评估空间学习和记忆。使用高效液相色谱（HPLC）测量海马和前额皮质中的γ-氨基丁酸（GABA）水平。结果显示，腹腔注射氯胺酮（30 mg/kg，每天两次）4周导致小鼠空间认知能力下降，而补充420 mg/（kg·d）DMA 6周在一定程度上改善了氯胺酮所致的空间认知障碍。海马和前额叶皮层 GABA 水平的上调与空间学习的改善有关。我们的结果表明，补充 DHA 将是改善氯胺酮引起的空间记忆和认知功能障碍的一种有前途的干预措施，并且 DHA 的这种作用可能与 GABA 水平的上调相关。 (C) 2014 Elsevier Ireland Ltd. 保留所有权利。
Several studies have reported the ketamine-induced cognitive impairment. Docosahexaenoic acid (DHA) supplementation improves cognitive function in human infants and protects against learning impairment in patients with Alzheimer's disease (AD). In this study, we investigated the effect of DMA on ketamine-induced impairment of spatial cognition and learning ability in Institute of Cancer Research (ICR) mice. Morris water maze (MWM) was used to assess spatial learning and memory. Gamma-aminobutyric acid (GABA) levels in the hippocampus and prefrontal cortex were measured using high-performance liquid chromatography (HPLC). The results showed that intraperitoneal injection of ketamine (30 mg/kg, twice per day) for 4 weeks led to the decline of spatial cognitive ability in mice, and 420 mg/(kg d) DMA supplementation for 6 weeks improved ketamine-induced spatial cognitive impairment to a certain extent. The up-regulation of GABA levels in the hippocampus and prefrontal cortex was related to the improvement in spatial learning. Our results suggested that DHA supplementation would be a promising intervention to improve ketamine-induced spatial memory and cognitive dysfunction, and this effect of DHA might be correlated with the up-regulation of GABA levels. (C) 2014 Elsevier Ireland Ltd. All rights reserved.