Interactions between mgr, asp, and polo:: asp function modulated by polo and needed to maintain the poles of monopolar and bipolar spindles
Interactions between mgr, asp, and polo:: asp function modulated by polo and needed to maintain the poles of monopolar and bipolar spindles
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DOI：
10.1007/s004120050329
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发表时间：
1998-12-01
期刊：
影响因子：
1.6
通讯作者：
Glover, DM
中科院分区：
文献类型：
--
作者：
Gonzalez, C;Sunkel, CE;Glover, DM
我们描述了基因突变之间的相互作用，在Mgr，ASP，和波罗，基因所需的正确行为的纺锤杆在果蝇。波罗（I）mgr双突变体的表型与mgr比波罗（I）更相似，但单独使用任一突变体时所见的圆形单极图（CMF）频率是相加的，这表明这两种基因产物在双极纺锤体形成中是独立功能所必需的。asp（E3）mgr双突变体在发育中比单独的任一突变体早得多地停滞，表明对细胞增殖的强烈阻断。我们讨论是否在这些细胞中的微管结构的缺乏反映了延长有丝分裂停滞，或者如果它是一个更直接的后果的双突变体组合。波罗（1）asp（E3）双突变体显示出有丝分裂频率的显著协同增加。通常与波罗（I）表型相关的CMFs的丢失表明，Asp微管相关蛋白是维持纺锤体极结构所必需的。推测Asp蛋白可能是波罗编码的丝氨酸-苏氨酸蛋白激酶的底物。
We describe genetic interactions between mutations in mgr, asp, and polo, genes required for the correct behaviour of the spindle poles in Drosophila. The phenotype of a polo(I) mgr double mutant is more similar to mgr than polo(I), but the frequency of circular monopolar figures (CMFs) seen with either mutant alone is additive, suggesting that the two gene products are required for independent functions in the formation of bipolar spindles. The asp(E3) mgr double mutant arrests much earlier in development than either mutant alone, indicative of a strong block to cell proliferation. We discuss whether the lack of microtubular structures in these cells reflects an extended mitotic arrest, or if it is a more direct consequence of the double mutant combination. A polo(1) asp(E3) double mutant shows a dramatic synergistic increase in mitotic frequency. The loss of CMFs normally associated with the polo(I)phenotype suggests that the Asp microtubule-associated protein is required to maintain the structure of spindle poles. We speculate that Asp protein might be a substrate for the serine-threonine protein kinase encoded by polo.