DETECTION OF ANTIBODY TO AUTOLOGOUS HUMAN LEUKEMIA-CELLS BY IMMUNE ADHERENCE ASSAYS
DETECTION OF ANTIBODY TO AUTOLOGOUS HUMAN LEUKEMIA-CELLS BY IMMUNE ADHERENCE ASSAYS
复制标题
DOI：
10.1073/pnas.74.10.4587
复制
发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
OLD, LJ
中科院分区：
文献类型：
--
作者：
GARRETT, TJ;TAKAHASHI, T;OLD, LJ
本文对21例成人急性白血病患者血清中与自体白血病细胞表面抗原反应的抗体进行了研究。从患者中获得连续的血清样品，并在免疫粘附测定中对冷冻保存的白血病细胞进行测试。3例患者显示自体血清反应性，并对其中1例患者的血清进行了详细分析。该抗体与自体急性淋巴细胞白血病细胞反应，但不与临床缓解期间从外周血、骨髓或脾脏获得的自体细胞反应。在吸收试验中，在正常自体或同种异体血液淋巴细胞、T-[胸腺衍生]或B-[骨髓衍生]细胞来源的淋巴母细胞系或感染猿猴肉瘤病毒、狒狒C型病毒或Mason-Pfizer病毒的细胞上均未检测到抗原。另外2例急性淋巴细胞白血病和1例急性非淋巴细胞白血病患者的白血病细胞吸收了特异性反应性。显然，某些急性白血病细胞表达一种共同抗原，在自体宿主中激发体液免疫应答。
The sera of 21 adult patients with acute leukemia were studied for the presence of antibody reacting with surface antigens of autologous leukemia cells. Sequential serum samples were obtained from patients and were tested on cryopreserved leukemia cells in immune adherence assays. Three patients showed autologous serum reactivity and the serum of 1 of them was analyzed in detail. This antibody reacted with autologous acute lymphocytic leukemia cells but not with autologous cells obtained from peripheral blood, bone marrow or spleen during clinical remission. In absorption tests, the antigen could not be detected on normal autologous or allogeneic blood lymphocytes, lymphoblastoid lines of T-[thymus-derived] or B-[bone marrow-derived] cell origin, or cells infected with simian sarcoma virus, baboon C-type virus or Mason-Pfizer virus. Leukemia cells from 2 other patients with acute lymphocytic leukemia and 1 patient with acute nonlymphocytic leukemia absorbed specific reactivity. Apparently, certain acute leukemia cells express a common antigen that elicits a humoral immune response in the autologous host.