First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance.
First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance.
复制标题
DOI：
10.3389/fmicb.2022.1036351
复制
发表时间：
2022
影响因子：
5.2
通讯作者：
Xu, Ying-Chun
中科院分区：
文献类型：
--
作者：
Chen, Xin-Fei;Hou, Xin;Zhang, Han;Jia, Xin-Miao;Ning, Li-Ping;Cao, Wei;Fan, Xin;Huang, Jing-Jing;Yang, Wen-Hang;Zhang, Ge;Zhang, Jing-Jia;Kang, Wei;Xiao, Meng;Xu, Ying-Chun
关键词：
海毛念珠菌变种VULNERA是哈穆罗尼弧菌的一种罕见变种，此前已有报道称该菌会导致人类感染。由于海默罗氏隐翅虫与变种海默罗氏隐翅虫的亲缘关系较近，因此，它们之间的亲缘关系十分密切。VULNIA，准确鉴定哈穆罗尼变种。VULNIA依赖于以rDNA内转录间隔区(ITS)为靶点的DNA测序分析。在这项工作中，两个品系的哈默罗尼变种。漏洞采集自中国医院侵袭性真菌监测网(CHIF-NET)。3种基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF MS)和VITEK 2 YST ID生化方法的鉴定能力与其测序结果进行了比较。此外，使用Sensititre YeastOne进行了抗真菌药敏试验。此外，我们还通过全基因组测序对耐药相关基因进行了全面筛选。利用MALDI-TOF MS和YST ID卡未能将这两个菌株正确地鉴定到物种变异水平。两株菌均对两性霉素B耐药(最低抑菌浓度为2μg/ml)。此外，菌株F4564和F4584对氟康唑(>；256μg/ml)和5-氟胞嘧啶(>；μg/ml)分别表现出较高的MIC值，这可能是由于Erg11p中的关键氨基酸Y132F和G307A以及Fur1p中的V58fs和G60K的关键氨基酸替换所致。稀有种海穆罗尼变种C.hamilonii var.中国身上出现了漏洞，这种可能导致侵袭性疾病的耐药真菌物种需要进一步密切关注。
Candida haemulonii var. vulnera is a rare variant of C. haemulonii, which has been previously reported to cause human infections. Owing to the close kinship between C. haemulonii sensu stricto and C. haemulonii var. vulnera, accurate identification of C. haemulonii var. vulnera relied on DNA sequencing assay targeting, for example, rDNA internal transcribed spacer (ITS) region. In this work, two strains of C. haemulonii var. vulnera were collected from the China Hospital Invasive Fungal Surveillance Net (CHIF-NET). The identification capacity of three matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and VITEK 2 YST ID biochemical methods were evaluated against ITS sequencing. In addition, antifungal susceptibility testing was performed using Sensititre YeastOne. Moreover, we comprehensively screened drug-resistant related genes by whole-genome sequencing. The two strains were not correctly identified to species variant level using MALDI-TOF MS and YST ID cards. Both strains were resistant to amphotericin B (minimum inhibitory concentration [MIC] > 2 μg/ml). Moreover, strain F4564 and F4584 exhibited high MIC to fluconazole (>256 μg/ml) and 5-flucytosine (>64 μg/ml), respectively, which were supposed to result from key amino acid substitutions Y132F and G307A in Erg11p and V58fs and G60K substitutions in Fur1p. The rare species C. haemulonii var. vulnera has emerged in China, and such drug-resistant fungal species that can cause invasive diseases require further close attention.
登录
查看更多内容
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
6.4
作者：
Arendrup, Maiken Cavling;Patterson, Thomas F.
通讯作者：
Patterson, Thomas F.
影响因子：
9.4
作者：
Pfaller, M. A.;Espinel-Ingroff, A.;Szeszs, M. W.
通讯作者：
Szeszs, M. W.
影响因子：
11.8
作者：
de Almeida JN Jr;Assy JG;Levin AS;Del Negro GM;Giudice MC;Tringoni MP;Thomaz DY;Motta AL;Abdala E;Pierroti LC;Strabelli T;Munhoz AL;Rossi F;Benard G
通讯作者：
Benard G
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro