Identification and characterization of foliar polyphenolic composition in sweetpotato (Ipomoea batatas L.) genotypes

Identification and characterization of foliar polyphenolic composition in sweetpotato (Ipomoea batatas L.) genotypes
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DOI:
10.1021/jf020120l
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发表时间:
2002-06-19
影响因子:
6.1
通讯作者:
Yamakawa, O
Yamakawa, O
中科院分区:
农林科学1区
文献类型:
--
作者:
Islam, MS;Yoshimoto, M;Yamakawa, O

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利用从世界各地收集的1389种甘薯(Ipomoea batatas L.)基因型进行了为期两年的试验,以表征甘薯叶片中的多酚成分。叶片总多酚成分和单叶多酚成分存在较大差异。在所有基因型中,甘薯叶片的总多酚含量为1.42 ~ 17.1 g/100 g干重。通过NMR, FABMS和RPHPLC分析程序对六种不同的多酚化合物进行鉴定和定量。这是甘薯叶片中多酚类成分的首次报道。甘薯叶片中多酚酸的相对含量为:3,5-二- o -咖啡酰奎宁酸,4,5-二- o -咖啡酰奎宁酸,>绿原酸(3- o -咖啡酰奎宁酸),> 3,4-二- o -咖啡酰奎宁酸,> 3,4,5-三- o -咖啡酰奎宁酸,>咖啡酸。3,4,5-三-咖啡酰奎宁酸和4,5-二- o -咖啡酰奎宁酸分别在221和1183.30 mg/100 g干重时最高。
Trials over two years were conducted using 1389 sweetpotato (Ipomoea batatas L.) genotypes collected from all over the world to characterize the polyphenolic composition in sweetpotato leaves. Wide variation was observed in relation to their total and individual leaf polyphenolic constituents. In all genotypes studied, the total polyphenol contents of sweetpotato leaf ranged from 1.42 to 17.1 g/100 g dry weight. The six different polyphenolic compounds were identified and quantified by NMR, FABMS, and RPHPLC analysis procedures. This is the first report of polyphenolic compositions in sweetpotato leaves. The relative levels of polyphenolic acids in sweetpotato leaves were as follows: 3,5-di-O-caffeoylquinic acid > 4,5-di-O-caffeoylquinic acid > chlorogenic acid (3-O-caffeoylquinic acid) > 3,4-di-O-caffeoylquinic acid > 3,4,5-tri-O-caffeoylquinic acid > caffeic acid. The highest 3,4,5-triO-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid occurred at 221 and 1183.30 mg/100 g dry weight, respectively.