Leg dysfunctions in a hatched chick model of spina bifida aperta
Leg dysfunctions in a hatched chick model of spina bifida aperta
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DOI：
10.1016/j.expneurol.2005.09.001
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发表时间：
2006-01-01
影响因子：
5.3
通讯作者：
Matsuda, S
中科院分区：
文献类型：
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作者：
Mominoki, K;Kinutani, M;Matsuda, S
我们在Hamburger-Hamilton阶段18或19通过切割胚胎神经管顶板制造了患有脊柱裂(SBA)的雏鸡。切开三个体节的长度导致47%的孵化雏鸡出现脊柱裂(SBO)样畸形。超过5个和7个体节长度的切开可导致100%的幼体出现SBA样畸形。SBO雏鸡没有表现出任何症状，而SBA雏鸡则表现出腿部肌肉瘫痪和所有激动剂和所有拮抗剂腿部肌肉之间的失衡。这些SBA雏鸡的损伤位于脊髓节段，这些节段产生了支配功能障碍肌肉的运动神经元。组织学分析表明，SBA雏鸡病变部位的小神经元(中间神经元)较正常鸡少，而大神经元(运动神经元)的数量无明显差异。这种SBA模型的腿功能障碍可能是由于脊髓节段中含有支配功能障碍肌肉的运动神经元的中间神经元数量较少所致。该模型有助于研究导致SBA雏鸡腿部功能障碍的病理机制。(C)2005 Elsevier Inc.保留所有权利。
We created chicks with spina bifida aperta (SBA) by incising the roof plate of the neural tube of embryos at Hamburger Hamilton stage 18 or 19. Incision over the length of three somites caused spina bifida occulta (SBO)-like malformation in 47% of the hatchlings. Incision over the length of five and seven somites caused SBA-like malformation in 100% of the hatchlings. The SBO chicks exhibited no symptoms, whereas the SBA chicks exhibited paralysis of a leg muscle and imbalance between all agonist and all antagonist leg muscles. Lesions in these SBA chicks were located in the spinal segments that give rise to motor neurons that innervated the dysfunctional muscles. Histological analysis revealed that there were fewer small spinal neurons (interneurons) at the site of the lesion in SBA chicks than in the normal chicks and that there was no such difference in the number of the large spinal neurons (motor neurons). Leg dysfunctions in this model of SBA may be attributable to the smaller number of interneurons in the spinal segments that contain motor neurons that innervate the dysfunctional muscle. This model may facilitate studies of the pathological mechanisms that lead to leg dysfunctions in SBA chicks. (c) 2005 Elsevier Inc. All rights reserved.