Thermoresponsive Poly(vinyl methyl ether) (PVME) Retained by 3-Aminopropyltriethoxysilane (APTES) Network
Thermoresponsive Poly(vinyl methyl ether) (PVME) Retained by 3-Aminopropyltriethoxysilane (APTES) Network
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由 3-氨基丙基三乙氧基硅烷 (APTES) 网络保留的热响应性聚（乙烯基甲基醚）(PVME)
DOI：
10.1021/acsbiomaterials.0c01376
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发表时间：
2020
影响因子：
5.8
通讯作者：
Zhang Newby, Bi-min
中科院分区：
文献类型：
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作者：
Malekzadeh, Elham;Zhang Newby, Bi-min
热响应聚合物（TRP）广泛应用于从控制分离中的膜污染到再生医学中的细胞/细胞片收获等各个方面。虽然聚（n -异丙基丙烯酰胺）（pNIPAAm）是最常用的TRP，但价格较低且易于加工的聚（乙烯基甲基醚）（PVME）在32-35℃时也表现出亲水性到疏水性的转变，接近生理条件。在这项研究中，我们研究了通过3-氨基丙基三乙基氧基硅烷（APTES）网络包埋在二氧化硅表面上保持稳定的PVME薄膜层的工艺条件。此外，还对保留的PVME薄膜的热响应行为（TRB）进行了评价。将质量比分别为90:10和50:50的PVME/APTES混合薄膜在室温条件下从其溶液中自旋涂覆，然后在40、60、80或120°C的真空烘箱中退火1、2或3天。然后用室温水彻底冲洗退火后的薄膜，然后在水中浸泡3天。我们的研究结果表明，在≥40℃的温度下退火是保持PVME膜在表面上的必要条件。较高的退火温度导致更大的薄膜保留，可能是由于形成了更紧密的APTES网络。无论加工条件如何，所有保留的PVME薄膜都表现出TRB，这是由低于和高于PVME转变温度的水接触角决定的。此外，在室温下，颗粒附着和蛋白质吸附在残留的PVME薄膜上的附着或吸附比在37℃时更低，并且在90:10的共混物中观察到更多PVME的差异更大。此外，人间充质干细胞在37℃下附着并增殖于保留的PVME表面，并在室温下迅速分离。这些结果说明了PVME表面作为低污染应用和非侵入性细胞收集的热响应支撑的潜在应用。
Thermoresponsive polymers (TRP)s have been widely used for various applications from controlling membrane fouling in separation to cell/cell sheet harvesting in regenerative medicine. While poly(N-isopropylacrylamide) (pNIPAAm) is the most commonly used TRP, less expensive and easily processed poly(vinyl methyl ether) (PVME) also shows a hydrophilic to hydrophobic transition at 32–35 °C, near physiological conditions. In this study, we investigated the processing conditions for retaining a stable layer of PVME thin film on silica surfaces via entrapment in a 3-aminopropyltriethoxysilane (APTES) network. In addition, the thermoresponsive behaviors (TRB) of the retained PVME films were evaluated. Blend thin films of PVME/APTES with 90:10 and 50:50 mass ratios were spin-coated from their solutions in ethanol under ambient conditions and then annealed in a vacuum oven at 40, 60, 80, or 120 °C for 1, 2, or 3 days. The annealed films were then thoroughly rinsed with room temperature water and then soaked in water for 3 days. Our results showed that annealing at a temperature of ≥40 °C was necessary for retaining a PVME film on the surface. The higher annealing temperature led to greater film retention, probably due to the formation of a tighter APTES network. Regardless of processing conditions, all retained PVME films showed TRB, determined by water contact angles below and above the transition temperature of PVME. Additionally, particle attachment and protein adsorption on retained PVME films showed lower attachment or adsorption at room temperature as compared to that at 37 °C, and a greater difference was observed for the 90:10 blend where more PVME was consisted. Furthermore, human mesenchymal stem cells attached and proliferated on the retained PVME surfaces at 37 °C and rapidly detached at room temperature. These results illustrated the potential applications of PVME surfaces as thermoresponsive supports for low-fouling applications and noninvasive cell harvesting.