HEAVY AND LIGHT PARTICLES OF ADENO-ASSOCIATED VIRUS
HEAVY AND LIGHT PARTICLES OF ADENO-ASSOCIATED VIRUS
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DOI：
10.1128/jvi.33.3.1129-1137.1980
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发表时间：
1980-01-01
影响因子：
5.4
通讯作者：
CARTER, BJ
中科院分区：
文献类型：
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作者：
DELAMAZA, LM;CARTER, BJ
用腺病毒和腺相关病毒(AAV)共感染的KB细胞产生了两种感染性AAV颗粒，分别以1.45g/cm2和1.41g/cm2的密度聚集在CSCL中。1.45条带被发现是由一组不同种类的病毒颗粒组成的，这些病毒颗粒可以通过速度沉降进行细分。这条谱带的主要成分的S值(109s)比1.41s的主要成分(111s)小，尽管两者具有相同的DNA/蛋白质比和相同的甲草胺密度梯度。连续标记实验表明，感染后早期，两个组分(1.45和1.41)的生成量相同，但随后1.41组分的比例相对于1.45颗粒的比例相对增加。脉冲追逐分析未能证明这两条带之间的前体-产物关系。在CSCL中，1.45频段的慢沉积成分不稳定，在感染后早期出现的比例较大。这些颗粒含有富含AAV基因组末端序列的DNA，并可被微球菌核酸酶消化。
KB cells coinfected with adenovirus and adeno-associated virus (AAV) yielded two kinds of infectious AAV particles that banded in CsCl at densities of 1.45 and 1.41 g/cm2, respectively. The 1.45 band was found to be composed of a heterogeneous group of viral particles that could be subfractionated by velocity sedimentation. The main component from this band had a smaller S value (109) than the main component from the 1.41 band (111S), although both had the same DNA/protein ratio and the same density in metrizamide gradients. Continuous-label experiments showed that early after infection, both components (1.45 and 1.41) were generated in the same amounts, but this was followed by a relative increase in the proportion of the 1.41 component over the 1.45 particles. Pulse-chase analysis failed to demonstrate a precursor-product relationship between these two bands. The slower-sedimenting components from the 1.45 band were unstable in CsCl and were present in a greater proportion early after infection. These particles contained DNA that was enriched for the terminal sequences of the AAV genomes and was accessible to digestion with micrococcal nuclease.