Isolation and identification of proangiotensin-12, a possible component of the renin-angiotensin system
Isolation and identification of proangiotensin-12, a possible component of the renin-angiotensin system
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DOI：
10.1016/j.bbrc.2006.09.146
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发表时间：
2006-12-01
影响因子：
3.1
通讯作者：
Kitamura, Kazuo
中科院分区：
文献类型：
--
作者：
Nagata, Sayaka;Kato, Johji;Kitamura, Kazuo
肾素-血管紧张素（RA）系统在调节血压和体液平衡中起着重要作用。为了寻找与血管紧张素II (Ang II) n端结合的抗体具有生物活性的肽，我们从大鼠小肠中分离出一种新的血管紧张素原衍生肽。该肽由12个氨基酸组成，基于其可能的Ang II前体作用，被称为血管紧张素-12。血管紧张素-12使大鼠主动脉条收缩，静脉注射后血压升高，而卡托普利和angii型受体阻滞剂CV-11974均能消除血管紧张素-12对血管的收缩和升压反应。血管紧张素-12原在大鼠的小肠、脾脏、肾脏和肝脏等多种器官和组织中含量丰富。血管紧张素-12原的鉴定表明RA系统的加工级联，不同于肾素将血管紧张素原裂解为Ang I。(c) 2006年Elsevier Inc.出版
The renin-angiotensin (RA) system plays an important role in regulating blood pressure and fluid balance. In the search for bioactive peptides with an antibody binding to the N-terminal portion of angiotensin II (Ang II), we isolated a new angiotensinogen-derived peptide from the rat small intestine. Consisting of 12 amino acids, this peptide was termed proangiotensin-12 based on its possible role of an Ang II precursor. Proangiotensin-12 constricted aortic strips and, when infused intravenously, raised blood pressure in rats, while both the vasoconstrictor and pressor response to proangiotensin-12 were abolished by captopril and by CV-11974, an Ang II type I receptor blocker. Proangiotensin-12 is abundant in a wide range of organs and tissues including the small intestine, spleen, kidneys, and liver of rats. The identification of proangiotensin-12 suggests a processing cascade of the RA system, different from the cleavage of angiotensinogen to Ang I by renin. (c) 2006 Published by Elsevier Inc.