Fluorescent in situ hybridization en suspension (FISHES) using digoxigenin-labeled probes and flow cytometry.
Fluorescent in situ hybridization en suspension (FISHES) using digoxigenin-labeled probes and flow cytometry.
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DOI：
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发表时间：
1992
期刊：
影响因子：
2.7
通讯作者：
E. A. Timm;C. Stewart
中科院分区：
文献类型：
--
作者：
E. A. Timm;C. Stewart
Fluorescent in situ hybridization has become a major technique for visualizing genetic material in fixed cells. Currently, many systems utilize the hybridization of labeled molecular probes to cells that are attached to slides. We have developed a technique that allows for in situ hybridization to be performed using cells in suspension. By using digoxigenin-labeled DNA probes and a fluoresceinated antibody directed against the digoxigenin, we can measure the resulting signal on a flow cytometer and the cells can be attached to microscope slides for visual analysis.
