Processing, Export, and Identification of Novel Linear Peptides from Staphylococcus aureus.
Processing, Export, and Identification of Novel Linear Peptides from Staphylococcus aureus.
复制标题
金黄色葡萄球菌新型线性肽的加工、出口和鉴定。
DOI：
10.1128/mbio.00112-20
复制
发表时间：
2020
期刊：
影响因子：
6.4
通讯作者：
Horswill,AlexanderR
中科院分区：
文献类型：
--
作者：
Schilcher,Katrin;Caesar,LindsayK;Cech,NadjaB;Horswill,AlexanderR
Staphylococcus aureus can colonize the human host and cause a variety of superficial and invasive infections. The success of S. aureus as a pathogen derives from its ability to modulate its virulence through the release, sensing of and response to cyclic signaling peptides. Here we provide, for the first time, evidence that S. aureus processes and secretes small linear peptides through a specialized pathway that converts a lipoprotein leader into an extracellular peptide signal. We have identified and confirmed the machinery for each step and demonstrate that the putative membrane metalloprotease Eep and the EcsAB transporter are required to complete the processing and secretion of the peptides. In addition, we have identified several linear peptides, including the interspecies signaling moleculestaph-cAM373, that are dependent on this processing and secretion pathway. These findings are particularly important because multiple Gram-positive bacteria rely on small linear peptides to control bacterial gene expression and virulence.IMPORTANCEHere, we provide evidence indicating that S. aureus secretes small linear peptides into the environment via a novel processing and secretion pathway. The discovery of a specialized pathway for the production of small linear peptides and the identification of these peptides leads to several important questions regarding their role in S. aureus biology, most interestingly, their potential to act as signaling molecules. The observations in this study provide a foundation for further in-depth studies into the biological activity of small linear peptides in S. aureus.
