Engineering Tumors: A Tissue Engineering Perspective in Cancer Biology
Engineering Tumors: A Tissue Engineering Perspective in Cancer Biology
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DOI：
10.1089/ten.teb.2009.0676
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发表时间：
2010-06-01
影响因子：
6.4
通讯作者：
Ludwig, Joseph A.
中科院分区：
文献类型：
--
作者：
Burdett, Emily;Kasper, F. Kurtis;Ludwig, Joseph A.
人们越来越认识到，三维(3D)组织培养技术在生物医学科学中的许多用途超出了再生医学的范围。其中一个用途是在癌症生物学领域，准确再现体内肿瘤表型的3D肿瘤模型将是研究肿瘤生物学的宝贵工具，并将允许更好地对抗癌候选药物进行临床前评估。最广泛使用的模型涉及被称为球体的小细胞聚集体，癌症生物学家使用这种聚集体已有数十年，并一直显示出3D组织培养在模拟体内遇到的肿瘤行为和耐药性方面优于标准的二维单层培养。目前，几个研究小组已经开始采用组织工程领域更先进的3D培养技术，以创建更准确的临床肿瘤生物学体外模型。
There is increasing recognition that three-dimensional (3D) tissue culture technologies have many uses within the biomedical sciences beyond the scope of regenerative medicine. One such use is in the field of cancer biology, where a 3D tumor model that accurately recreates the in vivo tumor phenotype would be a valuable tool for studying tumor biology and would allow better preclinical evaluation of anticancer drug candidates. The most widely used model involves small cellular aggregates, termed spheroids, which have been used by cancer biologists for decades and have consistently shown the superiority of 3D tissue culture over standard two-dimensional monolayer culture for mimicking the tumor behavior and drug resistance encountered in vivo. Currently, several research groups have begun to adapt more advanced 3D culture techniques from the tissue engineering field to create a more clinically accurate ex vivo model of tumor biology.