Biodegradation of malachite green by Kocuria rosea MTCC 1532
Biodegradation of malachite green by Kocuria rosea MTCC 1532
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DOI：
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发表时间：
2006-12
影响因子：
1.2
通讯作者：
G. Parshetti;S. Kalme;G. Saratale;S. Govindwar
中科院分区：
文献类型：
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作者：
G. Parshetti;S. Kalme;G. Saratale;S. Govindwar
在静态缺氧条件下，50 mg/L的孔雀石绿色在5 h内被玫瑰库克菌MTCC 1532完全脱色，而在振荡条件下没有脱色效果。K. Rosea还显示出对偶氮、三苯甲烷和工业染料（棉蓝、甲基橙子、活性蓝25、直接蓝-6、活性黄81和红HE 4 B）的脱色。含糖蜜、尿素和蔗糖的半合成培养基的脱色率分别为100%、91%和81%。孔雀石绿绿色还原酶和DCIP还原酶的活性在MG脱色过程中被诱导，表明它们参与了脱色过程。紫外-可见吸收光谱、高效液相色谱和红外光谱分析表明MG降解。毒性研究表明，MG被K.玫瑰色。
Malachite green (50 mg/L) was completely decolorized under static anoxic condition within 5 h by bacteria Kocuria rosea MTCC 1532; however decolorization was not observed at shaking condition. K. rosea have also shown decolorization of azo, triphenylmethane and industrial dyes (cotton blue, methyl orange, reactive blue 25, direct blue-6, reactive yellow 81, and red HE4B). Semi-synthetic media containing molasses, urea and sucrose have shown 100, 91, 81% decolorization respectively. Induction in the activities of malachite green reductase and DCIP reductase was observed during MG decolorization suggesting their involvement in the decolorization process. UV-Visible absorption spectrum, HPLC and FTIR analysis showed degradation of MG. Toxicity study revealed the degradation of MG into non-toxic products by K. rosea.