Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events.
Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events.
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DOI：
10.2147/cmar.s316599
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发表时间：
2021
影响因子：
3.3
通讯作者：
Zou F
中科院分区：
文献类型：
--
作者：
Zhou H;Zou M;Ding X;Zou F
肾上腺皮质癌(ACC)是一种少见、预后差的恶性肿瘤，人们对其诊断和治疗有进一步的研究兴趣。我们的研究旨在确定ACC的新的潜在治疗靶点。核心基因CDK1和CCNB1先前利用癌症基因组图谱(TCGA)的ACC数据进行了筛选，认为它们与Bclaf1和肿瘤预后最相关。我们使用基于siRNA或shRNA的模型来探讨Bclaf1在SW-13细胞系中的作用。用Western blotting和qPCR方法检测Bclaf1对CDK1和Cyclin B1的影响。基于生物信息分析，我们发现Bclaf1(Bclaf1)影响ACC的进展，与细胞周期有关。下调的Bclaf1表达抑制了Sw-13细胞的增殖，影响了Sw-13细胞的细胞周期进程。BCLAF1与CDK1和CCNB1相关，可调节它们的mRNA和蛋白水平。Bclaf1可能通过调节CDK1和Cyclin B1来促进有丝分裂，从而促进ACC的发展。
Adrenocortical carcinoma (ACC) is a rare malignancy with poor prognosis, and researchers are interested in further studying its diagnosis and treatment. Our study aims to identify new potential therapeutic targets in ACC. The core genes CDK1 and CCNB1 were previously screened using ACC data from The Cancer Genome Atlas (TCGA) as the most relevant to Bclaf1 and tumour prognosis. We used siRNA- or shRNA-based models to explore the role of Bcl-2-associated transcription factor 1 (Bclaf1) in SW-13 cell lines. Western blotting and qPCR were used to determine the effects of Bclaf1 on CDK1 and Cyclin B1. Based on biological information analysis, we found that Bcl-2-associated transcription factor 1 (Bclaf1) affected the progression of ACC and was associated with the cell cycle. Downregulated Bclaf1 expression inhibited the proliferation of SW-13 cells and affected the cell cycle process of SW-13 cells. BCLAF1 was correlated with CDK1 and CCNB1 and can regulate their mRNA and protein levels. Bclaf1 might promote the development of ACC by regulating CDK1 and Cyclin B1 to drive mitosis.