EXPRESSION OF ANGIOTENSIN-CONVERTING ENZYME-ACTIVITY IN CULTURED PULMONARY-ARTERY ENDOTHELIAL-CELLS
EXPRESSION OF ANGIOTENSIN-CONVERTING ENZYME-ACTIVITY IN CULTURED PULMONARY-ARTERY ENDOTHELIAL-CELLS
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DOI：
10.1002/jcp.1041080307
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发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
SMITH, JR
中科院分区：
文献类型：
--
作者：
DELVECCHIO, PJ;SMITH, JR
血管紧张素转换酶（EC 3.4.15.1）是一种羧基末端二肽基肽酶。这种酶催化十肽血管紧张素I转化为八肽血管紧张素II。此外，酶分解代谢缓激肽。由于这些作用，这种酶在血压稳态中起着关键作用。许多研究人员已经证明了这种酶与内皮细胞有关，但对培养的内皮细胞中控制酶活性表达的因素知之甚少。我们已经证明，内皮细胞在培养中不表达大量的酶活性，直到几天后，由于细胞密度高，生长停止。这是很重要的，因为它证明了在培养中随着生长阶段功能的变化，以及非分裂内皮细胞和因损伤而分裂的细胞之间功能能力的可能差异。由于分化性状的密度依赖性表达似乎并不是内皮细胞所独有的，因此了解这一现象背后的机制可能为培养细胞表达分化性状提供一般性解释。
Angiotensin‐converting enzyme (EC 3.4.15.1) is a carboxyterminal dipeptidyl peptidase. The enzyme catalyzes the conversion of the decapeptide angiotensin I to the octapeptide angiotensin II. In addition, the enzyme catabolizes bradykinin. Because of these actions, the enzyme is of pivotal importance in blood pressure homeostasis. Numerous investigators have demonstrated the presence of the enzyme in association with endothelial cells but relatively little is known concerning the factors controlling the expression of enzyme activity by endothelial cells in culture. We have demonstrated that endothelial cells in culture do not express significant amounts of enzyme activity until several days after growth ceases due to high cell density. This is important because it demonstrates a change in function with stage of growth in culture and a possible difference in functional capabilities between nondividing endothelial cells and cells that are dividing in response to injury. Since density‐dependent expression of differentiated traits does not appear to be unique to endothelial cells an understanding of the mechanisms underlying this phenomenon may provide a general explanation for the expression of differentiated traits by cultured cells.