CpG-Oligodeoxynucleotide Treatment Protects against Ionizing Radiation-Induced Intestine Injury.
CpG-Oligodeoxynucleotide Treatment Protects against Ionizing Radiation-Induced Intestine Injury.
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CpG-寡脱氧核苷酸治疗可预防电离辐射引起的肠道损伤
DOI：
10.1371/journal.pone.0066586
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cai JM
中科院分区：
文献类型：
--
作者：
Zhang C;Ni J;Li BL;Gao F;Liu H;Liu W;Huang YJ;Cai JM
背景骨髓和肠道是电离辐射（IR）引起的损伤的主要部位。我们之前的研究表明，CpG-寡脱氧核苷酸 (ODN) 治疗可减轻 IR 引起的骨髓损伤，但其对肠道的影响尚不清楚。在本研究中，我们试图确定 CpG-ODN 是否对 IR 诱导的肠道损伤具有保护作用，如果有，则确定其作用机制。方法和结果 IR 后用 CpG-ODN 治疗小鼠。暴露后连续30天每天监测体重和存活率。通过微菌落存活测定评估存活肠隐窝的数量。采用TUNEL法和BrdU法测定隐窝内增殖细胞的数量和分布。采用免疫组化法检测隐窝中Bcl-2、Bax、caspase-3的表达。研究结果表明，用 CpG-ODN 治疗受辐射的小鼠可减少体重减轻，提高 30 天存活率，增强肠隐窝存活并维持隐窝细胞群增殖和再生。其原因可能与CpG-ODN上调Bcl-2蛋白的表达、下调Bax蛋白和caspase-3蛋白的表达有关。结论 CpG-ODN 通过增强肠隐窝存活、维持隐窝细胞增殖和再生来有效保护 IR 诱导的肠道损伤。其机制可能是CpG-ODN通过调节Bax、Bcl-2、caspase-3等凋亡相关蛋白的表达来抑制增殖细胞凋亡。
Background the bone marrow and the intestine are the major sites of ionizing radiation (IR)-induced injury. Our previous study demonstrated that CpG-oligodeoxynucleotide (ODN) treatment mitigated IR-induced bone marrow injury, but its effect on the intestine is not known. In this study, we sought to determine if CpG-ODN have protective effect on IR-induced intestine injury, and if so, to determine the mechanism of its effect. Methods and Findings Mice were treated with CpG-ODN after IR. The body weight and survival were daily monitored for 30 days consecutively after exposure. The number of surviving intestinal crypt was assessed by the microcolony survival assay. The number and the distribution of proliferating cell in crypt were evaluated by TUNEL assay and BrdU assay. The expression of Bcl-2, Bax and caspase-3 in crypt were analyzed by Immunohistochemistry assay. The findings showed that the treatment for irradiated mice with CpG-ODN diminished body weight loss, improved 30 days survival, enhanced intestinal crypts survival and maintained proliferating cell population and regeneration in crypt. The reason might involve that CpG-ODN up-regulated the expression of Bcl-2 protein and down-regulated the expression of Bax protein and caspase-3 protein. Conclusion CpG-ODN was effective in protection of IR-induced intestine injury by enhancing intestinal crypts survival and maintaining proliferating cell population and regeneration in crypt. The mechanism might be that CpG-ODN inhibits proliferating cell apoptosis through regulating the expression of apoptosis-related protein, such as Bax, Bcl-2 and caspase-3.
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DOI：
10.1080/09553007014550291
发表时间：
1970-01-01
期刊：
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY AND RELATED STUDIES IN PHYSICS CHEMISTRY AND MEDICINE
影响因子：
--
作者：
WITHERS, HR;ELKIND, MM
通讯作者：
ELKIND, MM
影响因子：
8
作者：
Coopersmith, CM;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
4.8
作者：
Wang, Yaohong;Srinivasan, Kamalakkannan;Khurana, Seema
通讯作者：
Khurana, Seema
影响因子：
4.6
作者：
Pedersen, G;Andresen, L;Brynskov, J
通讯作者：
Brynskov, J
影响因子：
24.5
作者：
Ciorba MA;Riehl TE;Rao MS;Moon C;Ee X;Nava GM;Walker MR;Marinshaw JM;Stappenbeck TS;Stenson WF
通讯作者：
Stenson WF