The balance between the novel protein target of wingless and the Drosophila rho-associated kinase pathway regulates planar cell polarity in the Drosophila wing
The balance between the novel protein target of wingless and the Drosophila rho-associated kinase pathway regulates planar cell polarity in the Drosophila wing
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DOI：
10.1534/genetics.106.069021
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发表时间：
2007-06-01
期刊：
影响因子：
3.3
通讯作者：
Yim, Jeongbin
中科院分区：
文献类型：
--
作者：
Chung, SeYeon;Kim, Sangjoon;Yim, Jeongbin
平面细胞极性（PCP）信号是由蛇形受体卷曲（Fz）介导，凌乱（Dsh）转导的。无翼（Wg）信号通路利用果蝇卷曲2 （DFz2）作为受体，在许多发育过程中也需要Dsh作为转导信号来调节细胞增殖和分化。在Dsh下游的Wg-和fz信号通路中，不同的通路被激活。最近，在果蝇中发现了一些基因，它们作为PCP信号传导的下游组分具有重要作用。它们包括小GTPase RhoA/Rho1，其下游效应物Drosophila rho-associated kinase (Drok)，以及一些生化功能尚不清楚的基因，如inturn （in）和fuzzy （fy）。RhoA和Drok提供了Fz/Dsh信号与肌动蛋白细胞骨架调节的联系。在这里，我们报告了通过增强子陷阱筛选鉴定无翅（低）的新基因靶标。在翅膀成像盘中，tow的表达受Wg信号的负调控，而low和Drok通路之间的平衡调节着翼毛的形态发生。低基因的功能丧失突变不会导致明显的表型。遗传相互作用和功能获得研究提供了证据，证明Tow作用于Fz/Dsh下游，并在限制翼细胞形成的毛发数量方面发挥作用。
Planar cell polarity (PCP) signaling is mediated by the serpentine receptor Frizzled (Fz) and transduced by Dishevelled (Dsh). Wingless (Wg) signaling utilizes Drosophila Frizzled 2 (DFz2) as a receptor and also requires Dsh for transducing signals to regulate cell proliferation and differentiation in many developmental contexts. Distinct pathways are activated downstream of Dsh in Wg- and Fz-signaling pathways. Recently, a number of genes, which have essential roles as downstream components of PCP signaling, have been identified in Drosophila. They include the small GTPase RhoA/Rho1, its downstream effector Drosophila rho-associated kinase (Drok), and a number of genes such as inturned (in) and fuzzy (fy), whose biochemical functions are unclear. RhoA and Drok provide a link from Fz/Dsh signaling to the modulation of actin cytoskeleton. Here we report the identification of the novel gene target of wingless (low) by enhancer trap screening. tow expression is negatively regulated by Wg signaling in wing imaginal discs, and the balance between low and the Drok pathway regulates wing-hair morphogenesis. A loss-of-function mutation in low does not result in a distinct phenotype. Genetic interaction and gain-of-function studies provide evidence that Tow acts downstream of Fz/Dsh and plays a role in restricting the number of hairs that wing cells form.