HUMAN SOLEUS MUSCLE - COMPARISON OF FIBER COMPOSITION AND ENZYME-ACTIVITIES WITH OTHER LEG MUSCLES
HUMAN SOLEUS MUSCLE - COMPARISON OF FIBER COMPOSITION AND ENZYME-ACTIVITIES WITH OTHER LEG MUSCLES
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DOI：
10.1007/bf00587415
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发表时间：
1974-01-01
影响因子：
4.5
通讯作者：
SALTIN, B
中科院分区：
文献类型：
--
作者：
GOLLNICK, PD;SJODIN, B;SALTIN, B
在人体骨骼肌中研究了纤维组成和酶谱之间的关系，所述酶谱是从体外酶活性估计的。比目鱼肌、腓肠肌和股外侧肌的样本通过活检正常受试者和非肌肉相关的普通外科手术患者获得。分析样品的纤维成分、磷酸化酶（a+B）、磷酸果糖激酶和肌酸磷酸激酶活性。根据碱性稳定肌原纤维腺苷三磷酸酶染色的组织化学显示，将纤维分为两种主要类型。这种染色与酶的活性有关，因此与纤维的收缩性有关。一种纤维类型缺乏（I型或慢缩纤维），另一种（II型或快缩纤维）含有这种酶。比目鱼肌主要含有慢颤（I型）纤维，所有受试者的平均值为80%（范围64 - 100%）。与此相反，腓肠肌和股外侧肌仅含有57%的慢肌纤维（范围34-82%）。糖酵解酶的活性测定，除了己糖激酶，主要是慢收缩与许多快收缩纤维的肌肉相比，这是一致的α甘油磷酸脱氢酶的肌肉组织化学染色模式。琥珀酸脱氢酶和肌酸磷酸激酶活性与纤维分布无关。
The relationship between fiber composition and enzyme profiles as estimated from in vitro enzyme activities has been studied in human skeletal muscle. Samples from the soleus, gastrocnemius, and vastus lateralis muscles were obtained both by biopsying normal subjects and from patients during nonmuscular related general surgery. The samples were analyzed for fiber composition, phosphorylase (a+b), phosphofructokinase, and creatine phosphokinase activities. The fibers were assigned to two major types based on the histochemical display of alkaline stable myofibrillar adenosine triphosphatase staining. This staining is related to the activity of the enzyme and thus the contractility of the fibers. One fibre type lacks (Type I or slow twitch fiber) and the other one (Type II or fast twitch fiber) contains this enzyme. The soleus muscle contained predominantly slow twitch (Type I) fibers with the mean for all subjects being 80% (range 64 to 100%). In contrast the gastrocnemius and vastus lateralis muscles only contained 57% slow twitch fibers (range 34–82%). The activities of the glycolytic enzymes assayed, except hexokinase, were lower in predominantly slow twitch as compared with muscle with many fast twitch fibers and this was consistent with muscle histochemical staining patterns for alpha glycerophosphate dehydrogenase. Succinate dehydrogenase and creatine phosphokinase activities were not related to fiber distribution.