Systematizing and Cloning of Genes Involved in the Cerebellar Cortex Circuit Development
Systematizing and Cloning of Genes Involved in the Cerebellar Cortex Circuit Development
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DOI：
10.1007/s11064-011-0398-1
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发表时间：
2011-07-01
影响因子：
4.4
通讯作者：
Yoshikawa, Fumio
中科院分区：
文献类型：
--
作者：
Furuichi, Teiichi;Shiraishi-Yamaguchi, Yoko;Yoshikawa, Fumio
哺乳动物的小脑皮层神经回路在出生后的发育阶段通过一系列重要的细胞事件来完成功能性神经回路结构的形成。遗传因素被认为对小脑发育至关重要。因此，在发育过程中分析潜在的转录组以了解小脑皮质回路的遗传蓝图是至关重要的。在这篇综述中，我们介绍了大量的时空基因表达数据的发育时间序列微阵列分析和原位杂交细胞mRNA定位，并创建一个神经信息学数据库，称为小脑发育转录组数据库。使用这个数据库，我们已经确定了数千个基因，这些基因被分为各种功能类别，并与相关的细胞发育阶段一致表达。我们还提出了小脑发育的分子机制的功能表征的几个确定的基因（Cupidin，p130Cas，非常KIND，CAPS2）负责不同的细胞事件的发展小脑颗粒细胞。总之，小脑发育过程中的基因表达谱表明，小脑皮层回路的发育归因于复杂但协调的转录组。
The cerebellar cortical circuit of mammals develops via a series of magnificent cellular events in the postnatal stage of development to accomplish the formation of functional circuit architectures. The contribution of genetic factors is thought to be crucial to cerebellar development. Therefore, it is essential to analyze the underlying transcriptome during development to understand the genetic blueprint of the cerebellar cortical circuit. In this review, we introduce the profiling of large numbers of spatiotemporal gene expression data obtained by developmental time-series microarray analyses and in situ hybridization cellular mRNA mapping, and the creation of a neuroinformatics database called the Cerebellar Development Transcriptome Database. Using this database, we have identified thousands of genes that are classified into various functional categories and are expressed coincidently with related cellular developmental stages. We have also suggested the molecular mechanisms of cerebellar development by functional characterization of several identified genes (Cupidin, p130Cas, very-KIND, CAPS2) responsible for distinct cellular events of developing cerebellar granule cells. Taken together, the gene expression profiling during the cerebellar development demonstrates that the development of cerebellar cortical circuit is attributed to the complex but orchestrated transcriptome.