Relationships among early European maize inbreds:: IV.: Genetic diversity revealed with AFLP markers and comparison with RFLP, RAPD, and pedigree data
Relationships among early European maize inbreds:: IV.: Genetic diversity revealed with AFLP markers and comparison with RFLP, RAPD, and pedigree data
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DOI：
10.2135/cropsci2000.403783x
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发表时间：
2000-05-01
期刊：
影响因子：
2.3
通讯作者：
Kuiper, M
中科院分区：
文献类型：
--
作者：
Lübberstedt, T;Melchinger, AE;Kuiper, M
51份优质玉米(Zea Mays L.)利用8对扩增片段长度多态(AFLP)标记组合，对早熟欧洲火石和凹陷杂交种群体的自交系进行了AFLP标记分析。我们的主要目的是(I)调查AFLP标记在这些材料中的变异量，(Ii)检验AFLP标记在将自交系分配到杂种群体中的有效性，以及(Iii)比较基于AFLP标记的遗传相似性(GS)与基于系谱数据的Malecot同源系数(Cf)以及基于限制性片段长度多态(RFLP)和随机扩增多态DNA(RAPD)数据的GS估计。8对AFLP引物组合共扩增出462条多态性条带。根据AFLP数据计算的不相关(f=0)线对之间的GS估计值在0.38到0.77之间。所有弗林特自交系和凹陷自交系对来自其他杂种优势群的品系的平均GS小于对来自同一杂种优势群的无关品系的平均一般配合力。对于混合起源的品系，平均GS与火石品系和凹陷品系的差异与根据家系确定的每个杂种类群的预期基因组比例一致。GS估计值的主要坐标分析导致了打火石和凹陷线的单独分组。RFLP和AFLP标记的f和GS估计值之间的相关性显著高于RAPD。对于相关的(f>；0)和不相关的(f=0)火石和凹陷品系，基于不同标记系统的GS估计值的相关性在RFLP和AFLP标记之间最接近。这项研究的结果证实了AFLP标记在(I)将自交系划分为杂种优势群和(Ii)揭示品系之间的系谱关系方面的有效性。
A set of 51 elite maize (Zea mays L.) inbred lines representative of the early-maturing European flint and dent heterotic groups were assayed for amplified fragment length polymorphisms (AFLP) markers using eight primer combinations. Our main objectives were to (i) investigate the amount of variation for AFLP markers in these materials, (ii) examine the usefulness of AFLP markers for assigning inbred lines to heterotic groups, and (iii) compare the genetic similarity (GS) based on AFLP markers with Malecot's coancestry coefficient Cf) based on pedigree data and with GS estimates based on restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNA (RAPD) data. The eight AFLP primer combinations yielded 462 polymorphic bands. GS estimates calculated from AFLP data ranged between 0.38 and 0.77 between unrelated (f = 0) pairs of lines. All flint and dent inbreds showed a smaller mean GS to lines from the other heterotic group than to unrelated lines from the same heterotic group. For lines of mixed origin, the difference in mean GS to flint lines and to dent lines was consistent with the expected genomic proportions from each heterotic group determined on the basis of pedigrees. Principle coordinate analysis of GS estimates resulted in a separate grouping of flint and dent lines. Correlations between f and GS estimates were substantially higher for RFLPs and AFLP markers than for RAPDs. Correlations of GS estimates based on different marker systems were closest between RFLP and AFLP markers both for related (f > 0) and unrelated (f = 0) pairs of flint and dent lines. Results from this study corroborate the usefulness of AFLP markers for (i) assigning inbreds into heterotic groups and (ii) revealing pedigree relationships among lines.