β-carotene modulates the immunological function of RAW264, a murine macrophage cell line, by enhancing the level of intracellular glutathione
β-carotene modulates the immunological function of RAW264, a murine macrophage cell line, by enhancing the level of intracellular glutathione
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DOI：
10.1271/bbb.60056
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发表时间：
2006-09-01
影响因子：
1.6
通讯作者：
Yamanishi, Rintaro
中科院分区：
文献类型：
--
作者：
Imamura, Tomomi;Bando, Noriko;Yamanishi, Rintaro
本文研究了β-胡萝卜素对小鼠巨噬细胞系RAW 264细胞氧化还原状态和免疫功能的影响。补充β-胡萝卜素的RAW 264细胞导致明显不一致的氧化还原指数：脂质过氧化作用增强，但细胞内氧化适度减弱。减弱的细胞内氧化通过谷胱甘肽的增加伴随着γ-谷氨酰-半胱氨酸合成酶（谷胱甘肽合成的限速酶）亚基的转录上调而得到证实。α-生育酚能抑制自由基引起的脂质过氧化反应，但不影响β-胡萝卜素引起的脂质过氧化反应，也不影响细胞内氧化还原状态。脂多糖刺激的RAW 264中IL-1 β和IL-12 p40的转录被β-胡萝卜素抑制，但不被α-生育酚抑制。提示β-胡萝卜素通过提高细胞内谷胱甘肽水平调节巨噬细胞内氧化还原状态，与巨噬细胞的免疫功能有关。
The activities of beta-carotene on redox status and the immune functions of RAW264 cells, a murine macrophage cell line, were investigated. Supplementation with beta-carotene for RAW264 cells resulted in apparently inconsistent redox indices: lipid peroxidation was enhanced but intracellular oxidation was moderately attenuated. Attenuated intracellular oxidation was endorsed by an increase in glutathione accompanied by up-regulated transcription of a subunit of gamma-glutamyl-cysteine synthetase, the rate-limiting enzyme for glutathione synthesis. a-Tocopherol, which can quench lipid peroxidation by free radical, neither inhibited that by beta-carotene nor influenced the intracellular redox status. Lipopolysaccharide-stimulated transcriptions of IL-1 beta and IL-12 p40 in RAW264 were inhibited by beta-carotene but not by a-tocopherol. These results indicate that beta-carotene, which can, modulate the intracellular redox status of macrophages by enhancing the level of intracellular glutathione, is related to the immune functions of macrophages.