Phototrophy by antenna-containing rhodopsin pumps in aquatic environments
Phototrophy by antenna-containing rhodopsin pumps in aquatic environments
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DOI：
10.1038/s41586-023-05774-6
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发表时间：
2023-03-01
期刊：
影响因子：
64.8
通讯作者：
Beja, Oded
中科院分区：
文献类型：
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作者：
Chazan, Ariel;Das, Ishita;Beja, Oded
从捕光酮类胡萝卜素的光驱动的质子泵xanthorhodopsins的能量转移先前已被证明在两个独特的情况下：极端嗜盐细菌1和陆地蓝藻2。试图找到类胡萝卜素结合和转移能量丰富的视紫红质质子泵3从海洋光异养生物迄今失败4 -6。在这里，我们检测到光能转移广泛的羟基化类胡萝卜素玉米黄质和叶黄素的视网膜部分的xanthorhodopsins和proteorhodopsins使用功能宏基因组学结合从环境中提取的发色团。捕光类胡萝卜素将在紫光或蓝光范围内收集的能量的42%转移到吸收绿光的视网膜发色团。我们的数据表明，这些天线可能对世界湖泊，海洋和海洋中的视紫红质光养作用产生重大影响。然而，我们的研究结果的功能意义还有待发现。
Energy transfer from light-harvesting ketocarotenoids to the light-driven proton pump xanthorhodopsins has been previously demonstrated in two unique cases: an extreme halophilic bacterium1 and a terrestrial cyanobacterium2. Attempts to find carotenoids that bind and transfer energy to abundant rhodopsin proton pumps3 from marine photoheterotrophs have thus far failed4-6. Here we detected light energy transfer from the widespread hydroxylated carotenoids zeaxanthin and lutein to the retinal moiety of xanthorhodopsins and proteorhodopsins using functional metagenomics combined with chromophore extraction from the environment. The light-harvesting carotenoids transfer up to 42% of the harvested energy in the violet or blue-light range to the green-light absorbing retinal chromophore. Our data suggest that these antennas may have a substantial effect on rhodopsin phototrophy in the world's lakes, seas and oceans. However, the functional implications of our findings are yet to be discovered.