Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine
Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine
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DOI：
10.1007/s10103-013-1369-3
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发表时间：
2014-03-01
影响因子：
2.1
通讯作者：
Gerbi, M. E. M.
中科院分区：
文献类型：
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作者：
Araujo, N. C.;Fontana, C. R.;Gerbi, M. E. M.
光动力疗法（PDT）是一种涉及在氧存在下通过光激活光敏剂的技术，导致能够诱导细胞死亡的反应性自由基的产生。本研究评价了变形链球菌和嗜酸乳杆菌在生物膜阶段与牙本质龋损中对PDT的敏感性。用添加1%葡萄糖、2%蔗糖和1%乳杆菌幼原代培养物的脑心浸液培养基。嗜酸乳杆菌10（8）CFU/mL;使用10（8）CFU/mL的变形杆菌在人牙本质板上形成多物种生物膜并诱导龋齿。使用五种不同浓度的姜黄素（0.75、1.5、3.0、4.0和5.0 g/L）与5.7 J/cm（2）发光二极管相关联。分析四个不同的组L-D-（对照组）、L-D+（药物组）、L+D-（光照组）和L+D+（PDT组）。进行ANOVA/Tukey检验以比较组。在所有测试的姜黄素浓度下，在光敏化后的生物膜相中观察到细胞活力的显著降低（p < 0.05）。为了在龋坏牙本质中实现显著的细菌减少（p < 0.05），有必要利用5.0g/L的姜黄素与蓝光联合。未发现L-D+的显著降低，支持不存在药物的暗毒性。S. mutans和L.嗜酸乳杆菌在蓝光存在下对姜黄素敏感。然而，由于光穿透和药物扩散的困难，这些微生物在牙本质龋损内的影响小于它们在生物膜阶段。
Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals that are capable of inducing cell death. The present study evaluated the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to PDT grown as multi-species in the biofilm phase versus in dentine carious lesions. A brain-heart infusion culture medium supplemented with 1 % glucose, 2 % sucrose, and 1 % young primary culture of L. acidophilus 10(8) CFU/mL and S. mutans 10(8) CFU/mL was used to develop multi-species biofilms and to induce caries on human dentine slabs. Five different concentrations of curcumin (0.75, 1.5, 3.0, 4.0, and 5.0 g/L) were used associated with 5.7 J/cm(2) light emission diode. Four different groups were analyzed L-D- (control group), L-D+ (drug group), L+D- (light group), and L+D+ (PDT group). ANOVA/Tukey's tests were conducted to compare groups. A significant reduction (p < 0.05) in cell viability was observed in the biofilm phase following photosensitization with all curcumin concentrations tested. To achieve significant bacterial reduction (p < 0.05) in carious dentine, it was necessary to utilize 5.0 g/L of curcumin in association with blue light. No significant reduction was found for L-D+, supporting the absence of the drug's dark toxicity. S. mutans and L. acidophilus were susceptible to curcumin in the presence of blue light. However, due to light penetration and drug diffusion difficulties, these microorganisms within dentine carious lesions were less affected than they were in the biofilm phase.